ArticleApplied and environmental microbiology2013
The evolutionary divergence of Shiga toxin-producing Escherichia coli is reflected in clustered regularly interspaced short palindromic repeat (CRISPR) spacer composition.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- TheEcoSal Plus · 2025Review
- Isolation, Identification, and Antibiotic Resistance, CRISPR System Analysis ofMicroorganisms · 2025Article
- Comprehensive Genomic Analysis of UropathogenicPathogens (Basel, Switzerland) · 2024Article
- Escherichia coli CRISPR arrays from early life fecal samples preferentially target prophages.The ISME journal · 2024Article
- Identification and characterization of two CRISPR/Cas systems associated with the mosquito microbiome.Access microbiology · 2023Article
- Large-Scale Phylogenetic Analysis Reveals a New Genetic Clade among Escherichia coli O26 Strains.Microbiology spectrum · 2022Article
- Distribution of CRISPR inAnimals : an open access journal from MDPI · 2022Article
- Genotyping Based on CRISPR Loci Diversity and Pathogenic Potential of DiarrheagenicFrontiers in microbiology · 2022Article
- CRISPR-Cas systems are present predominantly on chromosome and its relationship with MEGs in Vibrio species.Archives of microbiology · 2021Article
- Shining light on a deep-sea bacterial symbiont population structure with CRISPR.Microbial genomics · 2021Article
- Exploration of the Diversity of Clustered Regularly Interspaced Short Palindromic Repeats-Cas Systems inFrontiers in microbiology · 2021Article
- Preserving US microbe collections sparks future discoveries.Journal of applied microbiology · 2020Review
- Article
- Clinical and molecular characteristics of carbapenem non-susceptible Escherichia coli: A nationwide survey from Oman.PloS one · 2020Article
- Utilization of Clustered Regularly Interspaced Short Palindromic Repeats to GenotypeFrontiers in microbiology · 2020Article
- Genetic Diversity of Non-O157 Shiga Toxin-ProducingFrontiers in microbiology · 2020Article
- Article
- Complete Genome Sequence and Genomic Characterization ofFrontiers in pharmacology · 2018Article
- Suppressing the CRISPR/Cas adaptive immune system in bacterial infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Review
- Shiga Toxin-Producing Serogroup O91 Escherichia coli Strains Isolated from Food and Environmental Samples.Applied and environmental microbiology · 2017Article
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6 authors.
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Abstract
The Shiga toxin-producing Escherichia coli (STEC) strains, including those of O157:H7 and the "big six" serogroups (i.e., serogroups O26, O45, O103, O111, O121, and O145), are a group of pathogens designated food adulterants in the United States. The relatively conserved nature of clustered regularly interspaced short palindromic repeats (CRISPRs) in phylogenetically related E. coli strains makes them potential subtyping markers for STEC detection, and a quantitative PCR (qPCR)-based assay was previously developed for O26:H11, O45:H2, O103:H2, O111:H8, O121:H19, O145:H28, and O157:H7 isolates. To better evaluate the sensitivity and specificity of this qPCR method, the CRISPR loci of 252 O157 and big-six STEC isolates were sequenced and analyzed along with 563 CRISPR1 and 624 CRISPR2 sequences available in GenBank. General conservation of spacer content and order was observed within each O157 and big-six serogroup, validating the qPCR method. Meanwhile, it was found that spacer deletion, the presence of an insertion sequence, and distinct alleles within a serogroup are sources of false-negative reactions. Conservation of CRISPR arrays among isolates expressing the same flagellar antigen, specifically, H7, H2, and H11, suggested that these isolates share an ancestor and provided an explanation for the false positives previously observed in the qPCR results. An analysis of spacer distribution across E. coli strains provided limited evidence for temporal spacer acquisition. Conversely, comparison of CRISPR sequences between strains along the stepwise evolution of O157:H7 from its O55:H7 ancestor revealed that, over this ∼7,000-year span, spacer deletion was the primary force generating CRISPR diversity.
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