ArticleExperimental parasitology2013
Use of RNAlater as a preservation method for parasitic coprology studies in wild-living chimpanzees.
Article in Experimental parasitology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Evaluation of short-term hair follicle storage conditions for maintenance of RNA integrity.PloS one · 2024Article
- Suitability of RNALater solution as a tissue-preserving reagent for immunohistochemical analysis.Histochemistry and cell biology · 2019Article
- A fecal sequel: Testing the limits of a genetic assay for bat species identification.PloS one · 2019Article
- Ethanol as a potential mosquito sample storage medium for RNA preservation.F1000Research · 2019Article
- Lyophilized human cells stored at room temperature preserve multiple RNA species at excellent quality for RNA sequencing.Oncotarget · 2018Article
- Transcriptome and proteome responses in RNAlater preserved tissue of Arabidopsis thaliana.PloS one · 2017Article
- Article
- Assessment of gastrointestinal parasites in wild chimpanzees (Pan troglodytes troglodytes) in southeast Cameroon.Parasitology research · 2014Article
- Article
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Authors and funding
9 authors.
Funding
Abstract
We evaluated the use of an RNA stabilisation buffer, RNAlater (Ambion, Austin, Texas), as a preservation medium for parasitic coprology analysis of faecal samples collected from chimpanzees living in the wild (Pan troglodytes troglodytes). Thirty faecal samples collected in the forests of south-east Cameroon (Mambele area) from 2003 to 2011 were preserved in RNAlater at -80 °C and analysed for their parasite content. We identified and counted parasitic elements and assessed their shape, size and morphology in relation to the storage time of the samples. We found that parasite elements were identifiable in RNAlater preserved samples after as many as 7 years, showing that RNAlater could be an effective and reliable preservation medium for coprology. Thus, its use could be an interesting way to optimise sample collection for several types of studies (parasitology and bacteriology/virology) at once, especially considering the logistically challenging and time-consuming field campaigns needed to obtain these faecal samples.
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Registered trials
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