ArticleJournal of clinical microbiology2013
Persistence of DNA in a cured patient and positive culture in cases with low antibody levels bring into question diagnosis of Q fever endocarditis.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Blood Culture-Negative Endocarditis: A Scientific Statement From the American Heart Association:Journal of the American Heart Association · 2025Guideline
- Diagnostic Yield and Incremental Value of 16S rRNA Gene PCR and Sequencing on Excised Heart-Valve Tissue in Infective Endocarditis: A Systematic Review and Meta-Analysis.Diagnostics (Basel, Switzerland) · 2026Review
- Emerging Q Fever Infection in Türkiye.Journal of tropical medicine · 2026Article
- Advances in pathogen detection ofInfectious medicine · 2025Review
- CRISPR-Cas12 Application for the Detection ofInternational journal of molecular sciences · 2025Article
- Development and Evaluation of Rapid and Accurate CRISPR/Cas13-Based RNA Diagnostics forFrontiers in cellular and infection microbiology · 2022Article
- Blood Culture-Negative Cardiovascular Infection in a Patient With Multiple Sclerosis.Open forum infectious diseases · 2019Article
- FluorescenceJournal of clinical microbiology · 2018Article
- Molecular detection of Coxiella burnetii in heart valve tissue from patients with culture-negative infective endocarditis.Medicine · 2018Observational
- Article
- Bartonella henselae is usually not viable in lymph nodes of patients with cat scratch disease.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- Article
- From Q Fever to Coxiella burnetii Infection: a Paradigm Change.Clinical microbiology reviews · 2017Review
- Subacute, tetracycline-responsive, granulomatous osteomyelitis in an adult man, consistent with Q fever infection.BMJ case reports · 2015Article
- Bartonella, a common cause of endocarditis: a report on 106 cases and review.Journal of clinical microbiology · 2015Review
- Specific in vitro interferon-gamma and IL-2 production as biomarkers during treatment of chronic Q fever.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We evaluated the performance of tools for diagnosing Q fever cardiovascular infection. We retrospectively analyzed 162 cardiovascular samples from 125 patients who were tested serologically by immunofluorescence, quantitative PCR (qPCR), 16S rRNA gene amplification, culture, and immunohistochemistry, and we assessed the viability of Coxiella burnetii by measuring the transcription of the 16S rRNA gene. The qPCR technique was significantly more sensitive than 16S rRNA gene amplification (P < 0.0001), cell culture (P = 0.0002), and immunohistochemistry (P < 0.0001). The sensitivity of these techniques was reduced when applied to patients who had been previously treated. The severity of infection appears to be correlated with phase I IgG levels. We report for the first time 4 cases of endocarditis with positive qPCR and/or culture assay result from patients with a low phase I IgG (IgG I) titer (<800), and we have identified the longest (16 years) persistence of DNA described in a heart valve from a patient cured after being previously treated for endocarditis. The active transcription of the 16S rRNA gene was found in 19/59 tested samples, with a positive predictive value of 100% for a positive culture. In conclusion, the diagnosis of Q fever cardiovascular infection should not be excluded in patients with low titers of phase I IgG when they present with valvulopathy. We recommend testing cardiovascular samples using 3 or 4 different biopsy sections by qPCR evaluation for patients with IgG I titers of ≥200.
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