ArticleJournal of clinical microbiology2013
Sensitive and specific quantitative detection of rotavirus A by one-step real-time reverse transcription-PCR assay without antecedent double-stranded-RNA denaturation.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 72 citations in OpenAlex.
- The effect of diarrheal disease on bivalent oral polio vaccine (bOPV) immune response in infants in Nepal.Vaccine · 2016Trial
- First Isolation of Bovine Coronavirus From Yanbian, China, and Analytical Validation of a SYBR Green I RT-qPCR Panel for Calf Diarrhea Viruses.Transboundary and emerging diseases · 2026Article
- GC Content and Thermal Stability of Double-Stranded RNA: Fragments of MicrosporidiaInternational journal of molecular sciences · 2025Article
- Rotavirus Genotype Dynamics and the Emergence of G3P[8] in Thailand Following Nationwide Vaccine Implementation.International journal of molecular sciences · 2025Article
- Rotavirus prevalence and genotypes in the Central African Republic, 2011-2021.BMC infectious diseases · 2025Article
- Removal capacities of wastewater treatment plants to minimize spread of enteroviruses.Journal of water and health · 2025Article
- Article
- Correlates of Rotavirus Vaccine Shedding and Seroconversion in a US Cohort of Healthy Infants.The Journal of infectious diseases · 2024Article
- Evaluation of different genomic regions of rotavirus B and rotavirus C for development of real-time RT‒PCR assays.Virology journal · 2024Article
- Neutralizing Antibodies Impair the Oncolytic Efficacy of Reovirus but Permit Effective Combination with T cell-Based Immunotherapies.Cancer immunology research · 2024Article
- Development and application of one-step multiplex Real-Time PCR for detection of three main pathogens associated with bovine neonatal diarrhea.Frontiers in cellular and infection microbiology · 2024Article
- Article
- Intertumoral Differences Dictate the Outcome of TGF-β Blockade on the Efficacy of Viro-Immunotherapy.Cancer research communications · 2023Article
- Gastrointestinal cancer-associated fibroblasts expressing Junctional Adhesion Molecule-A are amenable to infection by oncolytic reovirus.Cancer gene therapy · 2022Article
- Diversity of rotavirus strains circulating in Haiti before and after introduction of monovalent vaccine.IJID regions · 2022Article
- Genotype distribution and evolutionary analysis of rotavirus associated with acute diarrhea outpatients in Hubei, China, 2013-2016.Virologica Sinica · 2022Article
- Preinduced reovirus-specific T-cell immunity enhances the anticancer efficacy of reovirus therapy.Journal for immunotherapy of cancer · 2022Article
- Case Report: Rotavirus Vaccination and Severe Combined Immunodeficiency in Japan.Frontiers in immunology · 2022Article
- Development of a Real-Time Reverse Transcription-PCR Assay To Detect and Quantify Group A Rotavirus Equine-Like G3 Strains.Journal of clinical microbiology · 2021Article
- Cross-sectional study and genotyping of rotavirus-A infections in ruminants in Kuwait.BMC veterinary research · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A real-time quantitative reverse transcription-PCR (qRT-PCR) assay using the recombinant thermostable Thermus thermophilus (rTth) enzyme was developed to detect and quantify rotavirus A (RVA). By using rTth polymerase, significant improvement was achieved over the existing real-time RT-PCR assays, which require denaturation of the RVA double-stranded RNA (dsRNA) prior to assay setup. Using a dsRNA transcript for segment 7, which encodes the assay target NSP3 gene, the limit of detection for the improved assay was calculated to be approximately 1 genome copy per reaction. The NSP3 qRT-PCR assay was validated using a panel of 1,906 stool samples, 23 reference RVA strains, and 14 nontarget enteric virus samples. The assay detected a diverse number of RVA genotypes and did not detect other enteric viruses, demonstrating analytical sensitivity and specificity for RVA in testing stool samples. A XenoRNA internal process control was introduced and detected in a multiplexed qRT-PCR format. Because it does not require an antecedent dsRNA denaturation step, this assay reduces the possibility of sample cross-contamination and requires less hands-on time than other published qRT-PCR protocols for RVA detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.