ArticleAnalytical biochemistry2013
Oxidized fatty acid analysis by charge-switch derivatization, selected reaction monitoring, and accurate mass quantitation.
Article in Analytical biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- Genetic deletion of skeletal muscle iPLAiScience · 2023Article
- Liquid Chromatography-Mass Spectrometry (LC-MS) Derivatization-Based Methods for the Determination of Fatty Acids in Biological Samples.Molecules (Basel, Switzerland) · 2022Review
- Cardiac immune cell infiltration associates with abnormal lipid metabolism.Frontiers in cardiovascular medicine · 2022Article
- Recent advances in analytical strategies for mass spectrometry-based lipidomics.Analytica chimica acta · 2020Review
- Enhancing detection and characterization of lipids using charge manipulation in electrospray ionization-tandem mass spectrometry.Chemistry and physics of lipids · 2020Review
- A functional role for eicosanoid-lysophospholipids in activating monocyte signaling.The Journal of biological chemistry · 2020Article
- The Cell Membrane ofInternational journal of molecular sciences · 2020Review
- 12-LOX catalyzes the oxidation of 2-arachidonoyl-lysolipids in platelets generating eicosanoid-lysolipids that are attenuated by iPLAThe Journal of biological chemistry · 2020Article
- Ferroptotic cell death and TLR4/Trif signaling initiate neutrophil recruitment after heart transplantation.The Journal of clinical investigation · 2019Article
- A Unique Collision-Induced Dissociation Reaction of Cholamine Derivatives of Certain Prostaglandins.Journal of the American Society for Mass Spectrometry · 2018Article
- Establishment of a Charge Reversal Derivatization Strategy to Improve the Ionization Efficiency of Limaprost and Investigation of the Fragmentation Patterns of Limaprost Derivatives Via Exclusive Neutral Loss and Survival Yield Method.Journal of the American Society for Mass Spectrometry · 2018Article
- Heart failure-induced activation of phospholipase iPLAThe Journal of biological chemistry · 2018Article
- The evolution of lipidomics through space and time.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- The phospholipase iPLAThe Journal of biological chemistry · 2017Article
- Assessment of Isoprostanes in Human Plasma: Technical Considerations and the Use of Mass Spectrometry.Lipids · 2016Review
- Cyclooxygenase-2 Mediated Oxidation of 2-Arachidonoyl-Lysophospholipids Identifies Unknown Lipid Signaling Pathways.Cell chemical biology · 2016Article
- Cardiac Myocyte-specific Knock-out of Calcium-independent Phospholipase A2γ (iPLA2γ) Decreases Oxidized Fatty Acids during Ischemia/Reperfusion and Reduces Infarct Size.The Journal of biological chemistry · 2016Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
A highly sensitive, specific, and robust method for the analysis of oxidized metabolites of linoleic acid (LA), arachidonic acid (AA), and docosahexaenoic acid (DHA) was developed using charge-switch derivatization, liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI MS/MS) with selected reaction monitoring (SRM) and quantitation by high mass accuracy analysis of product ions, thereby minimizing interferences from contaminating ions. Charge-switch derivatization of LA, AA, and DHA metabolites with N-(4-aminomethylphenyl)-pyridinium resulted in a 10- to 30-fold increase in ionization efficiency. Improved quantitation was accompanied by decreased false positive interferences through accurate mass measurements of diagnostic product ions during SRM transitions by ratiometric comparisons with stable isotope internal standards. The limits of quantitation were between 0.05 and 6.0pg, with a dynamic range of 3 to 4 orders of magnitude (correlation coefficient r(2)>0.99). This approach was used to quantitate the levels of representative fatty acid metabolites from wild-type (WT) and iPLA2γ(-/-) mouse liver identifying the role of iPLA2γ in hepatic lipid second messenger production. Collectively, these results demonstrate the utility of high mass accuracy product ion analysis in conjunction with charge-switch derivatization for the highly specific quantitation of diminutive amounts of LA, AA, and DHA metabolites in biologic systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.