ArticleFertility and sterility2013
Coculturing human endometrial epithelial cells and stromal fibroblasts alters cell-specific gene expression and cytokine production.
Article in Fertility and sterility, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed.
- Trial
- Engineering the human endometrium at the intersection of development and reproduction.Nature biomedical engineering · 2026Review
- Simvastatin and Primaquine Identified as Potential Endometriosis Therapeutics via a Novel Epithelial-Stromal Assembloid Drug Screening Assay.bioRxiv : the preprint server for biology · 2026Article
- Three-Dimensional Organoid-like Co-Culture of Human Endometrial Endothelial and Stromal Cells to Study Endometriosis-Associated Responses.International journal of molecular sciences · 2026Article
- Establishment and characterization of a new immortalized human adenomyosis epithelial-like cell line, tAEC21†.Biology of reproduction · 2026Article
- Engineering the Human Endometrial-Embryo Interface: Breakthroughs in 3D Uterine Models.Biomolecules · 2026Review
- Female Reproductive Tract Organ-on-Chips: Modeling Barrier Function and Drug Transport.Pharmaceutics · 2026Review
- Modelling Endometriosis Using In Vitro and In Vivo Systems.International journal of molecular sciences · 2025Review
- How telomere maintenance affects endometriosis development: a preliminary study.International journal of medical sciences · 2025Article
- Oviduct and endometrial epithelium improve in vitro produced bovine embryo developmental kinetics.Reproduction (Cambridge, England) · 2024Article
- Basolateral secretions of human endometrial epithelial organoids impact stromal cell decidualization.Molecular human reproduction · 2023Article
- MSX1 Regulates Goat Endometrial Function by Altering the Plasma Membrane Transformation of Endometrial Epithelium Cells during Early Pregnancy.International journal of molecular sciences · 2023Article
- In Silico, In Vitro, and In Vivo Analysis Identifies Endometrial Circadian Clock Genes in Recurrent Implantation Failure.The Journal of clinical endocrinology and metabolism · 2021Article
- Endometrial regeneration with endometrial epithelium: homologous orchestration with endometrial stroma as a feeder.Stem cell research & therapy · 2021Article
- Endometrial Stem/Progenitor Cells-Their Role in Endometrial Repair and Regeneration.Frontiers in reproductive health · 2021Review
- A 3D endometrial organotypic model simulating the acute inflammatory decidualisation initiation phase with epithelial induction of the key endometrial receptivity marker, integrin αVβ3.Human reproduction open · 2021Article
- Seminal plasma promotes decidualization of endometrial stromal fibroblasts in vitro from women with and without inflammatory disorders in a manner dependent on interleukin-11 signaling.Human reproduction (Oxford, England) · 2020Article
- Women with polycystic ovary syndrome present with altered endometrial expression of stanniocalcin-1†.Biology of reproduction · 2020Article
- In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration.Journal of assisted reproduction and genetics · 2018Article
- Regulation of inflammatory mediator expression in bovine endometrial cells: effects of lipopolysaccharide, interleukin 1 beta, and tumor necrosis factor alpha.Physiological reports · 2018Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
objectiveTo determine the effects of coculturing endometrial epithelial cells (eEC) with paired endometrial stromal fibroblasts (eSF) on cell-specific gene expression and cytokine secretion patterns.
designIn vitro study.
settingUniversity research laboratory. PATIENT(S): Endometrial biopsies were obtained from premenopausal women. INTERVENTION(S): Polarized eEC and subject-paired eSF were cultured for 12.5 hours alone (monoculture) or combined in a two-chamber coculture system without cell-cell contact. Cells and conditioned media were analyzed for global gene expression and cytokine secretion, respectively. Purified, endometrial tissue-derived eEC and eSF isolated by fluorescent activated cell sorting (FACS) were used as noncultured controls. MAIN OUTCOME MEASURE(S): Cell-specific global gene expression profiling and analysis of secreted cytokines in eEC/eSF cocultures and respective monocultures. RESULT(S): Transepithelial resistance, diffusible tracer exclusion, expression of tight junction proteins, and apical/basolateral vectorial secretion confirmed eEC structural and functional polarization. Distinct transcriptomes of eEC and eSF were consistent with their respective lineages and their endometrial origin. Coculture of eEC with eSF resulted in altered cell-specific gene expression and cytokine secretion. CONCLUSION(S): This coculture model provides evidence that interactions between endometrial functionally polarized epithelium and stromal fibroblasts affect cell-specific gene expression and cytokine secretion underscoring their relevance when modeling endometrium in vitro.
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