ArticlePlant methods2013
A Narcissus mosaic viral vector system for protein expression and flavonoid production.
Article in Plant methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Plant Viral Vectors for Vaccine Development.Vaccines · 2026Review
- A transient expression tool box for anthocyanin biosynthesis in Nicotiana benthamiana.Plant biotechnology journal · 2024Article
- Virus-induced overexpression of heterologous FLOWERING LOCUS T for efficient speed breeding in tomato.Journal of experimental botany · 2024Article
- Foxtail mosaic virus: A tool for gene function analysis in maize and other monocots.Molecular plant pathology · 2023Review
- Review
- Development and optimization of a pepino mosaic virus-based vector for rapid expression of heterologous proteins in plants.Applied microbiology and biotechnology · 2021Article
- A Versatile Plant Rhabdovirus-Based Vector for Gene Silencing, miRNA Expression and Depletion, and Antibody Production.Frontiers in plant science · 2020Article
- Development of Beet necrotic yellow vein virus-based vectors for multiple-gene expression and guide RNA delivery in plant genome editing.Plant biotechnology journal · 2019Article
- Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses.Plant biotechnology journal · 2019Review
- Article
- Rewiring carotenoid biosynthesis in plants using a viral vector.Scientific reports · 2017Article
- When plant virology met Agrobacterium: the rise of the deconstructed clones.Plant biotechnology journal · 2015Review
- Transient expressions of synthetic biology in plants.Current opinion in plant biology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWith the explosive numbers of sequences generated by next generation sequencing, the demand for high throughput screening to understand gene function has grown. Plant viral vectors have been widely used as tools in down-regulating plant gene expression. However, plant viral vectors can also express proteins in a very efficient manner and, therefore, can also serve as a valuable tool for characterizing proteins and their functions in metabolic pathways in planta.
resultsIn this study, we have developed a Gateway®-based high throughput viral vector cloning system from Narcissus Mosaic Virus (NMV). Using the reporter genes of GFP and GUS, and the plant genes PAP1 (an R2R3 MYB which activates the anthocyanin pathway) and selenium-binding protein 1 (SeBP), we show that NMV vectors and the model plant Nicotiana benthamiana can be used for efficient protein expression, protein subcellular localization and secondary metabolite production.
conclusionsOur results suggest that not only can the plant viral vector system be employed for protein work but also can potentially be amenable to producing valuable secondary metabolites on a large scale, as the system does not require plant regeneration from seed or calli, which are stages where certain secondary metabolites can interfere with development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.