ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2013
Weight loss and reduced body temperature determine humane endpoints in a mouse model of ocular herpesvirus infection.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Who cites it
32 citing papers in PubMed, 56 citations in OpenAlex.
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- The antidiabetic effect of safflower yellow by regulating the GOAT/ghrelin/GHS-R1a/cAMP/TRPM2 pathway.Scientific reports · 2025Article
- Infrared Thermography for Temperature Measurement in Adult Female C57BL/6NCrl Mice: A Comparison with Rectal Probe and Subcutaneous Transponder.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Using weight loss to predict outcome and define a humane endpoint in preclinical sepsis studies.Scientific reports · 2024Article
- Evidence-Based Severity Assessment of Animal Models for Pancreatic Cancer.Biomedicines · 2024Article
- Humane Endpoint: Example from a Murine Model of Disseminated Sporotrichosis.Current microbiology · 2024Article
- Key considerations to improve the normalization, interpretation and reproducibility of morbidity data in mammalian models of viral disease.Disease models & mechanisms · 2024Article
- A Novel Scoring System for Humane Endpoints in Mice with Cecal Ligation and Puncture-Induced Sepsis.Comparative medicine · 2023Article
- Body Temperature Drop as a Humane Endpoint in Snake Venom-Lethality Neutralization Tests.Toxins · 2023Article
- Increased Neutrophil Percentage and Neutrophil-T Cell Ratio Precedes Clinical Onset of Experimental Cerebral Malaria.International journal of molecular sciences · 2023Article
- A Simple Telemetry Sensor System for Monitoring Body Temperature in Rabbits-A Brief Report.Animals : an open access journal from MDPI · 2023Article
- Robustness of a multivariate composite score when evaluating distress of animal models for gastrointestinal diseases.Scientific reports · 2023Article
- Tumor Temperature: Friend or Foe of Virus-Based Cancer Immunotherapy.Biomedicines · 2022Review
- Effects of intramuscularly injected plant-derived antimicrobials in the mouse model.Scientific reports · 2022Article
- Article
- Drastic hypothermia after intraperitoneal injection of okadaic acid, a diarrhetic shellfish poisoning toxin, in mice.Experimental animals · 2021Article
- Automated and Continuous Monitoring of Animal Welfare through Digital Alerting.Comparative medicine · 2020Article
- Impaired Recovery from Influenza A/X-31(H3N2) Infection in Mice with 8-Lipoxygenase Deficiency.Medical sciences (Basel, Switzerland) · 2019Article
- Identifying and Implementing Endpoints for Geriatric Mice.Comparative medicine · 2018Article
- Effects of Rodent Thermoregulation on Animal Models in the Research Environment.Comparative medicine · 2018Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Herpes simplex virus (HSV) has been studied in well-established mouse models to generate latently infected animals for investigations into viral pathogenesis, latency mechanisms, and reactivation. Mice exhibit clinical signs of debilitating infection, during which time they may become severely ill before recovery or die spontaneously. Because the cohort of mice that does survive provides valuable data on latency, there is keen interest in developing methodologies for earlier detection and treatment of severe disease to ultimately increase survival rates. Here, BALB/c mice were inoculated ocularly with either a wildtype (LAT(+)) or mutant (LAT(-)) strain of HSV1. Mice were monitored daily through day 30 after infection; trigeminal ganglia were harvested at day 60 to assess viral DNA load. Cages were provided with nesting material, and fluid supplementation was administered to mice with body temperatures of 35 °C or lower, as measured by subcutaneous microchip thermometry. The results showed that infected mice with temperatures less than 34.5 °C did not recover to normothermia and were euthanized or spontaneously died, regardless of infective viral strain. By using a combination of criteria including body temperature (less than 34.5 °C) and weight loss (more than 0.05 g daily) for removal of animals from the study, approximately 98% of mice that died spontaneously could have been euthanized prior to death, without concern of potential recovery to the experimental endpoint (100% specificity). Frequent monitoring of alterations to general wellbeing, body temperature, and weight was crucial for establishing humane endpoints in this ocular HSV model.
Indexed as
Identifiers
23849410PMC3690449W46487352What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.