ArticleBiomedical optics express2013
Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination.
Article in Biomedical optics express, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Optical sectioning methods in three-dimensional bioimaging.Light, science & applications · 2025Review
- 3D super-resolution optical fluctuation imaging with temporal focusing two-photon excitation.Biomedical optics express · 2024Article
- Multiline orthogonal scanning temporal focusing (mosTF) microscopy for scattering reduction in in vivo brain imaging.Scientific reports · 2024Article
- Multiline Orthogonal Scanning Temporal Focusing (mosTF) Microscopy for Scattering Reduction in High-speedResearch square · 2023Article
- Cascaded domain multiphoton spatial frequency modulation imaging.Journal of biomedical optics · 2023Article
- Temporal focusing multiphoton microscopy with cross-modality multi-stage 3D U-Net for fast and clear bioimaging.Biomedical optics express · 2023Article
- Image improvement of temporal focusing multiphoton microscopy via superior spatial modulation excitation and Hilbert-Huang transform decomposition.Scientific reports · 2022Article
- De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media.Science advances · 2021Article
- Temporal focusing multiphoton microscopy with optimized parallel multiline scanning for fast biotissue imaging.Journal of biomedical optics · 2021Article
- Two-photon fluorescence imaging of subsurface tissue structures with volume holographic microscopy.Journal of biomedical optics · 2020Article
- Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens.Annual review of biomedical engineering · 2020Review
- Scanless two-photon excitation with temporal focusing.Nature methods · 2020Review
- Multiview microscopy of single cells through microstructure-based indirect optical manipulation.Biomedical optics express · 2020Article
- Demonstration of flat-top beam illumination in widefield multiphoton microscopy.Journal of biomedical optics · 2019Article
- Article
- Scattering reduction by structured light illumination in line-scanning temporal focusing microscopy.Biomedical optics express · 2018Article
- Fiber-bundle illumination: realizing high-degree time-multiplexed multifocal multiphoton microscopy with simplicity.Scientific reports · 2018Article
- Widefield compressive multiphoton microscopy.Optics letters · 2018Article
- High-speed 3D imaging of cellular activity in the brain using axially-extended beams and light sheets.Current opinion in neurobiology · 2018Review
- Four-dimensional light shaping: manipulating ultrafast spatiotemporal foci in space and time.Light, science & applications · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Although temporally focused wide-field two-photon microscopy (TFM) can perform depth resolved wide field imaging, it cannot avoid the image degradation due to scattering of excitation and emission photons when imaging in a turbid medium. Further, its axial resolution is inferior to standard point-scanning two-photon microscopy. We implemented a structured light illumination for TFM and have shown that it can effectively reject the out-of-focus scattered emission photons improving image contrast. Further, the depth resolution of the improved system is dictated by the spatial frequency of the structure light with the potential of attaining depth resolution better than point-scanning two-photon microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.