ArticleJournal of cancer science & therapy2012
In vitro assessment of oxidative stress and apoptotic mechanisms of garlic extract in the treatment of acute promyelocytic leukemia.
Article in Journal of cancer science & therapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Garlic Extract Triggers Cytotoxicity, DNA Damage, and Cell Cycle Arrest in Human Leukemic CellsJournal of nutrition & food sciences · 2025Article
- Fractionation and phytochemical composition of an ethanolic extract ofFrontiers in pharmacology · 2024Article
- Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction.Scientific reports · 2022Article
- Article
- Methods of Isolation of Active Substances from Garlic (Antioxidants (Basel, Switzerland) · 2022Review
- The Role of Bioactive Compounds in Natural Products Extracted from Plants in Cancer Treatment and Their Mechanisms Related to Anticancer Effects.Oxidative medicine and cellular longevity · 2022Review
- Effects ofMolecules (Basel, Switzerland) · 2021Article
- Article
- A Porcine Organ-Culture Glaucoma Model Mimicking Trabecular Meshwork Damage Using Oxidative Stress.Investigative ophthalmology & visual science · 2021Article
- Oxidative Stress Mediated Cytotoxicity, Cell Cycle Arrest, and Apoptosis Induced byOxidative medicine and cellular longevity · 2021Article
- Article
- Prostate Cancer Disparity, Chemoprevention, and Treatment by Specific Medicinal Plants.Nutrients · 2019Review
- Potential roles of reactive oxygen species derived from chemical substances involved in cancer development in the female reproductive system.BMB reports · 2018Review
- Oxidative Stress Modulation and ROS-Mediated Toxicity in Cancer: A Review onOxidative medicine and cellular longevity · 2017Review
- β-ESTRADIOL INDUCES CYTOTOXIC EFFECTS TO HUMAN T-LYMPHOMA (JURKAT) CELLS THROUGH OXIDATIVE STRESS.Journal of the Mississippi Academy of Sciences. Mississippi Academy of Sciences · 2015Article
- Dual effect of oxidative stress on leukemia cancer induction and treatment.Journal of experimental & clinical cancer research : CR · 2014Review
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
introductionGarlic supplementation in diet has been shown to be beneficial to cancer patients. Recently, its pharmacological role in the prevention and treatment of cancer has received increasing attention. However, the mechanisms by which garlic extract (GE) induces cytotoxicity, oxidative stress, and apoptosis in cancer cells remain largely unknown.
objectiveThe present study was designed to use HL-60 cells as a test model to evaluate whether or not GE-induced cytotoxicty and apoptosis in human leukemia (HL-60) cells is mediated through oxidative stress.
methodsHuman leukemia (HL-60) cells were treated with different concentrations of GE for 12 hr. Cell survival was determined by MTT assay. The extent of oxidative cell/tissue damage was determined by measuring malondialdehyde (lipid peroxidation biomarker) concentrations by spectrophotometry. Cell apoptosis was measured by flow cytometry assessment (Annexin-V and caspase-3 assays) and agarose gel electrophoresis (DNA laddering assay).
resultsData obtained from the MTT assay indicated that GE significantly (
conclusionOur finding indicates that GE-induced cytotoxicity and apoptosis in HL-60 cells involve phosphatidylserine externalization, caspase-3 activation, and nucleosomal DNA fragmentation associated with the formation of MDA, a by-product of lipid peroxidation and biomarker of oxidative stress. At therapeutic concentrations, GE-induced cytotoxic and apoptotic effects in HL-60 cells is mediated by oxidative stress.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.