ArticleInternational journal of biological sciences2013
Effects of cigarette smoke extracts on the growth and senescence of skin fibroblasts in vitro.
Article in International journal of biological sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- A cosmetic treatment based on the secretion of Cryptomphalus aspersa 40% improves the clinical results after the use of nonablative fractional laser in skin aging.Journal of cosmetic dermatology · 2020Trial
- Ashes to Rashes: An Exploration of the Intersection Between Smoking and Cutaneous Lupus Erythematosus.Biomolecules · 2025Review
- Role of ferroptosis-related IREB2 in the shared genetic etiology between smoking and facial aging: Insights from large-scale genome-wide cross-trait analysis.Computational and structural biotechnology journal · 2025Article
- Extracellular glucose triggers metabolic reprogramming of cultured human bronchial epithelial cells and indirect fibroblast activation.FEBS open bio · 2024Article
- Correlation between cigarette smoking and alcohol consumption and Rosacea: A two-sample Mendelian randomization study.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024Article
- Multidimensional assessment of the biological effects of electronic cigarettes on lung bronchial epithelial cells.Scientific reports · 2024Article
- Cutaneous Redox Senescence.Biomedicines · 2024Review
- The combination of cigarette smoke and solar rays causes effects similar to skin aging in a bilayer skin model.Scientific reports · 2023Article
- A cross-sectional study of Chinese women facial skin status with environmental factors and individual lifestyles.Scientific reports · 2022Article
- Senescence-Associated β-Galactosidase Detection in Pathology.Diagnostics (Basel, Switzerland) · 2022Review
- Facial Skin Aging Stages in Chinese Females.Frontiers in medicine · 2022Article
- The role of cancer stromal fibroblasts in mediating the effects of tobacco-induced cancer cell growth.Cancer cell international · 2021Review
- Skin Aging, Cellular Senescence and Natural Polyphenols.International journal of molecular sciences · 2021Review
- Signaling pathway perturbation analysis for assessment of biological impact of cigarette smoke on lung cells.Scientific reports · 2021Article
- Knockdown of insulin-like growth factor 2 gene disrupts mitochondrial functions in the liver.Journal of molecular cell biology · 2021Article
- The Protective Role of Bioactive Quinones in Stress-induced Senescence Phenotype of Endothelial Cells Exposed to Cigarette Smoke Extract.Antioxidants (Basel, Switzerland) · 2020Article
- tBHP treatment as a model for cellular senescence and pollution-induced skin aging.Mechanisms of ageing and development · 2020Article
- The Protective Effect of Aspirin Eugenol Ester on Paraquat-Induced Acute Liver Injury Rats.Frontiers in medicine · 2020Article
- Cigarette Smoke Induces Metabolic Reprogramming of the Tumor Stroma in Head and Neck Squamous Cell Carcinoma.Molecular cancer research : MCR · 2019Article
- Overexpressed vascular endothelial growth factor in adipose derived stem cells attenuates fibroblasts and skin injuries by ultraviolet radiation.Bioscience reports · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epidemiological studies have shown that cigarette smoke (CS), a very common environmental factor, plays an important role in skin aging. Although some in vivo studies have suggested that CS affects skin aging, the detailed effects of CS on skin cells in vitro remain largely unknown. In this study, we investigated the effects of cigarette smoke extract (CSE) on the growth, proliferation, and senescene of skin fibroblasts and the possible mechanism underlying these effects. Primary cultured human fibroblasts were exposed to a range of concentrations of CSE. Cell viability and cell proliferation after CSE exposure were analyzed with the methyl thiazolyl tetrazolium (MTT) assay and bromodeoxyuridine incorporation assay, respectively. Growth curves of fibroblasts exposed to different concentrations of CSE were developed and prolonged CSE-exposed cells were observed. Morphological and ultrastructural changes in fibroblasts were assessed by inverted light microscopy and transmission electron microscopy (TEM). Dying cells were stained with senescence-associated β-galactosidase (SA β-gal). Intracellular reactive oxygen species (ROS) levels, superoxide dismutase (SOD) activity, and glutathione peroxidase (GSH-Px) activity were determined by a colorimetric method. We found that proliferative capacity and growth were inhibited by CSE exposure in a dose- and time-dependent manner. Fibroblasts exposed to even low concentrations of CSE for a long period of time (5 passages) showed significantly increased SA β-gal activity and typical features of aging cells. Meanwhile, CSE inhibited superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and augmented ROS levels. Our observations suggest that CSE exposure impairs fibroblast growth and proliferation and leads to features similar to those seen in senescent cells. Oxidative stress injury and inhibition of antioxidant defense activity may be involved in CSE-induced fibroblast senescence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.