ArticleInvestigative ophthalmology & visual science2013
Quantitative profiling of major neutral lipid classes in human meibum by direct infusion electrospray ionization mass spectrometry.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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Who cites it
48 citing papers in PubMed, 69 citations in OpenAlex.
- Direct Correlation of Levels of Meibum Cholesteryl Esters, Hydrocarbon Chain Saturation, and Protein With Lipid Structure, in Relation to Age and Dry Eye.Investigative ophthalmology & visual science · 2026Article
- A Controlled-Area Droplet Evaporimeter Reveals Surface Pressure-Dependent Evaporation Resistance of the Tear Film Lipid Layer.Investigative ophthalmology & visual science · 2026Article
- Stability and Compressibility of Tear Film Lipid Layer: Impact of Benzalkonium Chloride Presence.bioRxiv : the preprint server for biology · 2026Article
- Comprehensive lipid analysis of human meibum and tears.Scientific reports · 2025Article
- One Step Closer to a Molecular Level Understanding of the Tear Film Lipid Layer by Surface X-ray Scattering.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Structural characterization of wax esters using ultraviolet photodissociation mass spectrometry.Analytical and bioanalytical chemistry · 2024Article
- Another Brick in the Wall of Tear Film Insights Added Through the Total Synthesis and Biophysical Profiling ofJournal of natural products · 2024Article
- Early-Stage Development of an Anti-Evaporative Liposomal Formulation for the Potential Treatment of Dry Eyes.ACS pharmacology & translational science · 2023Article
- Relationship Between Human Meibum Lipid Composition and the Severity of Meibomian Gland Dysfunction: A Spectroscopic Analysis.Investigative ophthalmology & visual science · 2023Article
- A Multimethodological Approach for the Chemical Characterization of Edible Insects: The Case Study ofFoods (Basel, Switzerland) · 2023Article
- Effect of Model Tear Film Lipid Layer on Water Evaporation.Investigative ophthalmology & visual science · 2023Article
- Biophysical properties of tear film lipid layer I. Surface tension and surface rheology.Biophysical journal · 2022Article
- Candidate Molecular Compounds as Potential Indicators for Meibomian Gland Dysfunction.Frontiers in medicine · 2022Review
- Human meibum and tear film derived cholesteryl and wax esters in meibomian gland dysfunction and tear film structure.The ocular surface · 2022Article
- A spectroscopic approach to measuring meibum lipid composition and conformation in donors with Sjӧgren's syndrome.Experimental eye research · 2021Article
- A spectroscopic study of the composition and conformation of cholesteryl and wax esters purified from meibum.Chemistry and physics of lipids · 2021Article
- Human Meibum and Tear Film Derived (O-Acyl)-Omega-Hydroxy Fatty Acids as Biomarkers of Tear Film Dynamics in Meibomian Gland Dysfunction and Dry Eye Disease.Investigative ophthalmology & visual science · 2021Article
- Human meibum and tear film derived (O-acyl)-omega-hydroxy fatty acids in meibomian gland dysfunction.The ocular surface · 2021Article
- Triacylglycerol lipidome from human meibomian gland epithelial cells: Description, response to culture conditions, and perspective on function.Experimental eye research · 2021Article
- Diverse meibum lipids produced by Awat1 and Awat2 are important for stabilizing tear film and protecting the ocular surface.iScience · 2021Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
purposeThe purpose of this investigation was to better understand lipid composition in human meibum.
methodsIntact lipids in meibum samples were detected by direct infusion electrospray ionization mass spectrometry (ESI-MS) analysis in positive detection mode using sodium iodide (NaI) as an additive. The peak intensities of all major types of lipid species, that is, wax esters (WEs), cholesteryl esters (CEs), and diesters (DEs) were corrected for peak overlapping and isotopic distribution; an additional ionization efficiency correction was performed for WEs and CEs, which was simplified by the observation that the corresponding ionization efficiency was primarily dependent on the specific lipid class and saturation degree of the lipids while independent of the carbon chain length. A set of WE and CE standards was spiked in meibum samples for ionization efficiency determination and absolute quantitation.
resultsThe absolute amount (μmol/mg) for each of 51 WEs and 31 CEs in meibum samples was determined. The summed masses for 51 WEs and 31 CEs accounted for 48 ± 4% and 40 ± 2%, respectively, of the total meibum lipids. The mass percentages of saturated and unsaturated species were determined to be 75 ± 2% and 25 ± 1% for CEs and 14 ± 1% and 86 ± 1% for WEs. The profiles for two types of DEs were also obtained, which include 42 α,ω Type II DEs, and 21 ω Type I-St DEs.
conclusionsMajor neutral lipid classes in meibum samples were quantitatively profiled by ESI-MS analysis with NaI additive.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.