ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2013
Relationship between DNA damage response, initiated by camptothecin or oxidative stress, and DNA replication, analyzed by quantitative 3D image analysis.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
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- Hydroxyurea-The Good, the Bad and the Ugly.Genes · 2021Review
- Functional regulation of the structure-specific endonuclease FEN1 by the human cytomegalovirus protein IE1 suggests a role for the re-initiation of stalled viral replication forks.PLoS pathogens · 2021Article
- Exploring microRNA Signatures of DNA Damage Response Using an Innovative System of Genotoxic Stress inFrontiers in plant science · 2021Article
- γH2AX in the S Phase after UV Irradiation Corresponds to DNA Replication and Does Not Report on the Extent of DNA Damage.Molecular and cellular biology · 2020Article
- PML-like subnuclear bodies, containing XRCC1, juxtaposed to DNA replication-based single-strand breaks.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants.Scientific reports · 2018Article
- Entry of Human Coronavirus NL63 into the Cell.Journal of virology · 2018Article
- High Dose Ascorbate Causes Both Genotoxic and Metabolic Stress in Glioma Cells.Antioxidants (Basel, Switzerland) · 2017Article
- Low level phosphorylation of histone H2AX on serine 139 (γH2AX) is not associated with DNA double-strand breaks.Oncotarget · 2016Article
- Suppression of STN1 enhances the cytotoxicity of chemotherapeutic agents in cancer cells by elevating DNA damage.Oncology letters · 2016Article
- Prompt repair of hydrogen peroxide-induced DNA lesions prevents catastrophic chromosomal fragmentation.DNA repair · 2016Article
- Spatial relationship between chromosomal domains in diploid and autotetraploid Arabidopsis thaliana nuclei.Nucleus (Austin, Tex.) · 2016Article
- Screening Nonionic Surfactants for Enhanced Biodegradation of Polycyclic Aromatic Hydrocarbons Remaining in Soil After Conventional Biological Treatment.Environmental science & technology · 2016Article
- Subnuclear localization, rates and effectiveness of UVC-induced unscheduled DNA synthesis visualized by fluorescence widefield, confocal and super-resolution microscopy.Cell cycle (Georgetown, Tex.) · 2016Article
- Review
- Activation of new replication foci under conditions of replication stress.Cell cycle (Georgetown, Tex.) · 2015Article
- Bleomycin-induced γH2AX foci map preferentially to replicating domains in CHO9 interphase nuclei.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2014Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
A method of quantitative analysis of spatial (3D) relationship between discrete nuclear events detected by confocal microscopy is described and applied in analysis of a dependence between sites of DNA damage signaling (γH2AX foci) and DNA replication (EdU incorporation) in cells subjected to treatments with camptothecin (Cpt) or hydrogen peroxide (H2O2). Cpt induces γH2AX foci, likely reporting formation of DNA double-strand breaks (DSBs), almost exclusively at sites of DNA replication. This finding is consistent with the known mechanism of induction of DSBs by DNA topoisomerase I (topo1) inhibitors at the sites of collisions of the moving replication forks with topo1-DNA "cleavable complexes" stabilized by Cpt. Whereas an increased level of H2AX histone phosphorylation is seen in S-phase of cells subjected to H2O2, only a minor proportion of γH2AX foci coincide with DNA replication sites. Thus, the increased level of H2AX phosphorylation induced by H2O2 is not a direct consequence of formation of DNA lesions at the sites of moving DNA replication forks. These data suggest that oxidative stress induced by H2O2 and formation of the primary H2O2-induced lesions (8-oxo-7,8-dihydroguanosine) inhibits replication globally and triggers formation of γH2AX at various distances from replication forks. Quantitative analysis of a frequency of DNA replication sites and γH2AX foci suggests also that stalling of replicating forks by Cpt leads to activation of new DNA replication origins. © 2013 International Society for Advancement of Cytometry.
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