ArticleDiagnostic molecular pathology : the American journal of surgical pathology, part B2013
Tandem duplication PCR: an ultrasensitive assay for the detection of internal tandem duplications of the FLT3 gene.
Article in Diagnostic molecular pathology : the American journal of surgical pathology, part B, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Clinical implications of molecular markers in acute myeloid leukemia.European journal of haematology · 2019Review
- FLT3-ITD and its current role in acute myeloid leukaemia.Medical oncology (Northwood, London, England) · 2017Review
- A Novel Tandem Duplication Assay to Detect Minimal Residual Disease in FLT3/ITD AML.Molecular diagnosis & therapy · 2015Article
- Minimal residual disease in acute myeloid leukemia--current status and future perspectives.Current hematologic malignancy reports · 2015Review
- Improved FLT3 internal tandem duplication PCR assay predicts outcome after allogeneic transplant for acute myeloid leukemia.Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation · 2014Article
- False positives in multiplex PCR-based next-generation sequencing have unique signatures.The Journal of molecular diagnostics : JMD · 2014Article
- Tumor cellularity as a quality assurance measure for accurate clinical detection of BRAF mutations in melanoma.Molecular diagnosis & therapy · 2014Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Internal tandem duplication (ITD) mutations of the FLT3 gene have been associated with a poor prognosis in acute myeloid leukemia. Detection of ITD-positive minor clones at the initial diagnosis and during the minimal residual disease stage may be essential. We previously designed a delta-PCR strategy to improve the sensitivity to 0.1% ITD-positive leukemia cells and showed that minor mutants with an allele burden of <1% can be clinically significant. In this study, we report on tandem duplication PCR (TD-PCR), a modified inverse PCR assay, and demonstrate a limit of detection of a few molecules of ITD mutants. The TD-PCR was initially designed to confirm ITD mutation of an amplicon, which was undetectable by capillary electrophoresis and was incidentally isolated by a molecular fraction collecting tool. Subsequently, TD-PCR detected ITD mutation in 2 of 77 patients previously reported as negative for ITD mutation by a standard PCR assay. TD-PCR can also potentially be applied to monitor minimal residual disease with high analytic sensitivity in a portion of ITD-positive acute myeloid leukemia patients. Further studies using TD-PCR to detect ITD mutants at diagnosis may clarify the clinical significance of those ITD mutants with extremely low allele burden.
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Registered trials
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