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A method for reversible drug delivery to internal tissues of Drosophila embryos.
Article in Fly. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- A novel needleless delivery of Cas9 ribonucleoprotein complex in Bemisia tabaci embryos for gene editing.World journal of microbiology & biotechnology · 2026Article
- Spatial organization of translation and translational repression in two phases of germ granules.Nature communications · 2024Article
- Multivalent interactions make adherens junction-cytoskeletal linkage robust during morphogenesis.The Journal of cell biology · 2021Article
- Exploiting the Potential ofBiomedicines · 2021Review
- Analysis of mitochondrial organization and function in the Drosophila blastoderm embryo.Scientific reports · 2017Article
- Cortical movement of Bicoid in early Drosophila embryos is actin- and microtubule-dependent and disagrees with the SDD diffusion model.PloS one · 2017Article
- Syndapin promotes pseudocleavage furrow formation by actin organization in the syncytial Drosophila embryo.Molecular biology of the cell · 2016Article
- Imaging Approaches to Investigate Myonuclear Positioning in Drosophila.Methods in molecular biology (Clifton, N.J.) · 2016Article
- In vivo quantitative analysis of Talin turnover in response to force.Molecular biology of the cell · 2015Article
- Syd/JIP3 and JNK signaling are required for myonuclear positioning and muscle function.PLoS genetics · 2014Article
- The talin head domain reinforces integrin-mediated adhesion by promoting adhesion complex stability and clustering.PLoS genetics · 2014Article
- A method of permeabilization of Drosophila embryos for assays of small molecule activity.Journal of visualized experiments : JoVE · 2014Article
- Morphogenesis of the somatic musculature in Drosophila melanogaster.Wiley interdisciplinary reviews. Developmental biologyReview
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
Drosophila melanogaster is a powerful model organism to elucidate basic cellular mechanisms of development. Indeed, much of our understanding of genetic pathways comes from work in Drosophila. However, mutations in many critical genes cause early embryonic lethality; thus, it is difficult to study the role of proteins that are required for early fundamental processes during later embryonic stages. We have therefore developed a method to reversibly deliver drugs to internal tissues of stage 15-16 Drosophila embryos using a 1:1 combination of D-limonene and heptane (LH). Specifically, delivery of Nocodazole was shown to be effective as evidenced by the significant decrease in microtubule density seen in muscle cells. Following complete depolymerization of the microtubule cytoskeleton, removing the Nocodazole and washing for 10 min was sufficient for the microtubule network to be re-established, indicating that drug delivery is reversible. Additionally, the morphology of LH-treated embryos resembled that of untreated controls, and embryo viability post-treatment with LH was significantly increased compared with previously reported permeabilization techniques. These advances in embryo permeabilization provide a means to disrupt protein function in vivo with high temporally specificity, bypassing the complications associated with genetic disruptions as they relate to the study of late-stage developmental mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.