ArticleBMC infectious diseases2013
Detection of group a streptococcal pharyngitis by quantitative PCR.
Article in BMC infectious diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- Comparison of Centor and McIsaac scores in primary care: a meta-analysis over multiple thresholds.The British journal of general practice : the journal of the Royal College of General Practitioners · 2020Pooled it
- Streptococcus pyogenes Colonization in Children Aged 24-59 Months in the Gambia: Impact of Live Attenuated Influenza Vaccine and Associated Serological Responses.The Journal of infectious diseases · 2023Trial
- An emm-type specific qPCR to track bacterial load during experimental human Streptococcus pyogenes pharyngitis.BMC infectious diseases · 2021Trial
- Smartphone-based Detection of Group A Streptococcal Pharyngitis in Ugandan Children: A Pilot Study.The Pediatric infectious disease journal · 2026Article
- Molecular Methods Enhance the Detection of Pyoderma-Related Streptococcus pyogenes and emm-Type Distribution in Children.The Journal of infectious diseases · 2025Article
- Use of Polymerase Chain Reaction to Characterize the Etiology of Culture-Negative Empyema and Parapneumonic Effusion Among Alaska Native Children-2018-2023.Journal of the Pediatric Infectious Diseases Society · 2025Article
- Rheumatic Heart Disease Burden in Africa and the Need to Build Robust Infrastructure.JACC. Advances · 2024Review
- Development of a clinical metagenomics workflow for the diagnosis of wound infections.BMC medical genomics · 2024Article
- Transmission potential ofmSphere · 2024Article
- Efficacy of Alum-Adjuvanted Peptide and Carbohydrate Conjugate Vaccine Candidates against Group AVaccines · 2024Article
- Evaluation of a multiplex-qPCR for paediatric pleural empyema-An observational study in hospitalised children.PloS one · 2024Observational
- Group A β-hemolytic Streptococcal Pharyngitis: An Updated Review.Current pediatric reviews · 2024Review
- Characteristics, management and changing incidence of children with empyema in a paediatric intensive care unit.Journal of paediatrics and child health · 2022Article
- The Utility of Rapid Group A Streptococcus Molecular Testing Compared with Throat Culture for the Diagnosis of Group A Streptococcal Pharyngitis in a High-Incidence Rheumatic Fever Population.Journal of clinical microbiology · 2021Article
- Nasopharyngeal Isolates from a Cohort of Medical Students with or without Pharyngitis.Sultan Qaboos University medical journal · 2020Article
- Article
- Amoxicillin effect on bacterial load in group A streptococcal pharyngitis: comparison of single and multiple daily dosage regimens.BMC pediatrics · 2019Article
- A Controlled Human Infection Model of Group AmSphere · 2019Article
- Evaluation of the SD Bioline Strep A Ultra Test in Relation With Number of Colony Forming Units and Color Intensity.Annals of laboratory medicine · 2019Article
- Evaluation of Simplexa Group A Strep Direct Kit Compared to Hologic Group A Streptococcal Direct Assay for Detection of Group A Streptococcus in Throat Swabs.Journal of clinical microbiology · 2018Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGroup A streptococcus (GAS) is the most common bacterial cause of sore throat. School-age children bear the highest burden of GAS pharyngitis. Accurate diagnosis is difficult: the majority of sore throats are viral in origin, culture-based identification of GAS requires 24-48 hours, and up to 15% of children are asymptomatic throat carriers of GAS. The aim of this study was to develop a quantitative polymerase chain reaction (qPCR) assay for detecting GAS pharyngitis and assess its suitability for clinical diagnosis.
methodsPharyngeal swabs were collected from children aged 3-18 years (n = 91) and adults (n = 36) located in the Melbourne area who presented with sore throat. Six candidate PCR assays were screened using a panel of reference isolates, and two of these assays, targeting speB and spy1258, were developed into qPCR assays. The qPCR assays were compared to standard culture-based methods for their ability to detect GAS pharyngitis. GAS isolates from culture positive swabs underwent emm-typing. Clinical data were used to calculate McIsaac scores as an indicator of disease severity.
resultsTwenty-four of the 127 samples (18.9%) were culture-positive for GAS, and all were in children (26%). The speB qPCR had 100% sensitivity and 100% specificity compared with gold-standard culture, whereas the spy1258 qPCR had 87% sensitivity and 100% specificity. Nine different emm types were found, of which emm 89, 3, and 28 were most common. Bacterial load as measured by qPCR correlated with culture load. There were no associations between symptom severity as indicated by McIsaac scores and GAS bacterial load.
conclusionsThe speB qPCR displayed high sensitivity and specificity and may be a useful tool for GAS pharyngitis diagnosis and research.
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