ArticlePloS one2013
Calcium-binding capacity of centrin2 is required for linear POC5 assembly but not for nucleotide excision repair.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Exploring the Potential Roles ofInternational journal of molecular sciences · 2025Article
- CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity.The Journal of cell biology · 2023Article
- Article
- Mass Spectrometry-Based Structural Proteomics for Metal Ion/Protein Binding Studies.Biomolecules · 2022Review
- Role of Primary Cilia in Skeletal Disorders.Stem cells international · 2022Review
- Structural Basis for the Functional Diversity of Centrins: A Focus on Calcium Sensing Properties and Target Recognition.International journal of molecular sciences · 2021Review
- Sub-centrosomal mapping identifies augmin-γTuRC as part of a centriole-stabilizing scaffold.Nature communications · 2021Article
- Article
- 14-3-3 proteins mediate the localization of Centrin2 to centrosome.Journal of biosciences · 2019Article
- Adolescent idiopathic scoliosis associated POC5 mutation impairs cell cycle, cilia length and centrosome protein interactions.PloS one · 2019Article
- Modular elements of the TPR domain in the Mps1 N terminus differentially target Mps1 to the centrosome and kinetochore.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Xeroderma pigmentosum group C sensor: unprecedented recognition strategy and tight spatiotemporal regulation.Cellular and molecular life sciences : CMLS · 2016Review
- Global-genome Nucleotide Excision Repair Controlled by Ubiquitin/Sumo Modifiers.Frontiers in genetics · 2016Review
- Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function.Molecular biology of the cell · 2015Article
- Centrin2 regulates CP110 removal in primary cilium formation.The Journal of cell biology · 2015Article
- Functional variants of POC5 identified in patients with idiopathic scoliosis.The Journal of clinical investigation · 2015Article
- Centrosomes back in the limelight.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Centrosomes, the principal microtubule-organising centres in animal cells, contain centrins, small, conserved calcium-binding proteins unique to eukaryotes. Centrin2 binds to xeroderma pigmentosum group C protein (XPC), stabilising it, and its presence slightly increases nucleotide excision repair (NER) activity in vitro. In previous work, we deleted all three centrin isoforms present in chicken DT40 cells and observed delayed repair of UV-induced DNA lesions, but no centrosome abnormalities. Here, we explore how centrin2 controls NER. In the centrin null cells, we expressed centrin2 mutants that cannot bind calcium or that lack sites for phosphorylation by regulatory kinases. Expression of any of these mutants restored the UV sensitivity of centrin null cells to normal as effectively as expression of wild-type centrin. However, calcium-binding-deficient and T118A mutants showed greatly compromised localisation to centrosomes. XPC recruitment to laser-induced UV-like lesions was only slightly slower in centrin-deficient cells than in controls, and levels of XPC and its partner HRAD23B were unaffected by centrin deficiency. Interestingly, we found that overexpression of the centrin interactor POC5 leads to the assembly of linear, centrin-dependent structures that recruit other centrosomal proteins such as PCM-1 and NEDD1. Together, these observations suggest that assembly of centrins into complex structures requires calcium binding capacity, but that such assembly is not required for centrin activity in NER.
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