ArticlePloS one2013
RNA-Seq-based analysis of the physiologic cold shock-induced changes in Moraxella catarrhalis gene expression.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Comprehensive genomic analyses revealed the adaptation strategies ofFrontiers in microbiology · 2026Article
- Bacterial acetate metabolism and its influence on human epithelia.Emerging topics in life sciences · 2024Review
- Bacterial Stress Responses as Potential Targets in Overcoming Antibiotic Resistance.Microorganisms · 2022Review
- The transcription factor network of E. coli steers global responses to shifts in RNAP concentration.Nucleic acids research · 2022Article
- Gene Expression Regulation in Airway Pathogens: Importance for Otitis Media.Frontiers in cellular and infection microbiology · 2022Review
- The Molecular Determinants of Thermoadaptation: Methanococcales as a Case Study.Molecular biology and evolution · 2021Article
- Emerging Adaptive Strategies Under Temperature Fluctuations in a Laboratory Evolution Experiment ofFrontiers in microbiology · 2021Article
- Isolate Specific Cold Response ofFrontiers in microbiology · 2019Article
- Multidrug Efflux Pumps at the Crossroad between Antibiotic Resistance and Bacterial Virulence.Frontiers in microbiology · 2016Review
- Moraxella catarrhalis AcrAB-OprM efflux pump contributes to antimicrobial resistance and is enhanced during cold shock response.Antimicrobial agents and chemotherapy · 2015Article
- A Moraxella catarrhalis two-component signal transduction system necessary for growth in liquid media affects production of two lysozyme inhibitors.Infection and immunity · 2015Article
- Omics profiles used to evaluate the gene expression of Exiguobacterium antarcticum B7 during cold adaptation.BMC genomics · 2014Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMoraxella catarrhalis, a major nasopharyngeal pathogen of the human respiratory tract, is exposed to rapid downshifts of environmental temperature when humans breathe cold air. The prevalence of pharyngeal colonization and respiratory tract infections caused by M. catarrhalis is greatest in winter. We investigated how M. catarrhalis uses the physiologic exposure to cold air to regulate pivotal survival systems that may contribute to M. catarrhalis virulence.
resultsIn this study we used the RNA-seq techniques to quantitatively catalogue the transcriptome of M. catarrhalis exposed to a 26 °C cold shock or to continuous growth at 37 °C. Validation of RNA-seq data using quantitative RT-PCR analysis demonstrated the RNA-seq results to be highly reliable. We observed that a 26 °C cold shock induces the expression of genes that in other bacteria have been related to virulence a strong induction was observed for genes involved in high affinity phosphate transport and iron acquisition, indicating that M. catarrhalis makes a better use of both phosphate and iron resources after exposure to cold shock. We detected the induction of genes involved in nitrogen metabolism, as well as several outer membrane proteins, including ompA, m35-like porin and multidrug efflux pump (acrAB) indicating that M. catarrhalis remodels its membrane components in response to downshift of temperature. Furthermore, we demonstrate that a 26 °C cold shock enhances the induction of genes encoding the type IV pili that are essential for natural transformation, and increases the genetic competence of M. catarrhalis, which may facilitate the rapid spread and acquisition of novel virulence-associated genes.
conclusionCold shock at a physiologically relevant temperature of 26 °C induces in M. catarrhalis a complex of adaptive mechanisms that could convey novel pathogenic functions and may contribute to enhanced colonization and virulence.
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