ArticlePloS one2013
Development of low phytate rice by RNAi mediated seed-specific silencing of inositol 1,3,4,5,6-pentakisphosphate 2-kinase gene (IPK1).
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 155 citations in OpenAlex.
- Accelerating iron biofortification in millets: progress, challenges, and future prospects.Planta · 2026Review
- Genome-wide association studies for identification of QTLs and key candidate genes to improve grain quality in rice (Food chemistry. Molecular sciences · 2025Article
- Toward Nutrient-Rich Rice: Biofortification through Mineral Accumulation and Low Phytic Acid Content.Journal of agricultural and food chemistry · 2025Article
- Conditional knockdown of gene expression in plants via 3' UTR editing.Plant communications · 2025Article
- Biotechnological approaches to reduce the phytic acid content in millets to improve nutritional quality.Planta · 2024Review
- Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions.Applied biochemistry and biotechnology · 2024Review
- OsIPK1 frameshift mutations disturb phosphorus homeostasis and impair starch synthesis during grain filling in rice.Plant molecular biology · 2024Article
- An Overview of Targeted Genome Editing Strategies for Reducing the Biosynthesis of Phytic Acid: an Anti-nutrient in Crop Plants.Molecular biotechnology · 2024Review
- Mineral nutrients in plants under changing environments: A road to future food and nutrition security.The plant genome · 2023Review
- Prospects of genetics and breeding for low-phosphate tolerance: an integrated approach from soil to cell.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Review
- High-value pleiotropic genes for developing multiple stress-tolerant biofortified crops for 21st-century challenges.Heredity · 2022Article
- CRISPR/Cas9 mediated disruption ofJournal of advanced research · 2022Article
- Mining of Potential Gene Resources for Breeding Nutritionally Improved Maize.Plants (Basel, Switzerland) · 2022Review
- Genome-Wide Association Study of Phytic Acid in Wheat Grain Unravels Markers for Improving Biofortification.Frontiers in plant science · 2022Article
- Genetic manipulation of anti-nutritional factors in major crops for a sustainable diet in future.Frontiers in plant science · 2022Review
- A tale of two metals: Biofortification of rice grains with iron and zinc.Frontiers in plant science · 2022Review
- Identification and comprehensive analysis of MIPSs in Rosaceae and their expression under abiotic stresses in rose (Frontiers in plant science · 2022Article
- Research and Progress on the Mechanism of Iron Transfer and Accumulation in Rice Grains.Plants (Basel, Switzerland) · 2021Review
- Integrating a genome-wide association study with transcriptomic data to predict candidate genes and favourable haplotypes influencing Brassica napus seed phytate.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021Article
- Transcriptome analysis identifies differentially expressed genes in the progenies of a cross between two low phytic acid soybean mutants.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytic acid (InsP(6)) is considered to be the major source of phosphorus and inositol phosphates in most cereal grains. However, InsP(6) is not utilized efficiently by monogastric animals due to lack of phytase enzyme. Furthermore, due to its ability to chelate mineral cations, phytic acid is considered to be an antinutrient that renders these minerals unavailable for absorption. In view of these facts, reducing the phytic acid content in cereal grains is a desired goal for the genetic improvement of several crops. In the present study, we report the RNAi-mediated seed-specific silencing (using the Oleosin18 promoter) of the IPK1 gene, which catalyzes the last step of phytic acid biosynthesis in rice. The presence of the transgene cassette in the resulting transgenic plants was confirmed by molecular analysis, indicating the stable integration of the transgene. The subsequent T4 transgenic seeds revealed 3.85-fold down-regulation in IPK1 transcripts, which correlated to a significant reduction in phytate levels and a concomitant increase in the amount of inorganic phosphate (Pi). The low-phytate rice seeds also accumulated 1.8-fold more iron in the endosperm due to the decreased phytic acid levels. No negative effects were observed on seed germination or in any of the agronomic traits examined. The results provide evidence that silencing of IPK1 gene can mediate a substantial reduction in seed phytate levels without hampering the growth and development of transgenic rice plants.
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