ArticlePloS one2013
Pig α1-acid glycoprotein: characterization and first description in any species as a negative acute phase protein.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Selection of a suitable animal model to evaluate secretion of drugs in the human milk: a systematic approach.Xenobiotica; the fate of foreign compounds in biological systems · 2024Article
- Pig Acute Phase Proteins as Non-Antibody Systemic Biomarkers of Intracellular Infections.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Proteomic methods for the study of porcine acute phase proteins - anything new to detect?Veterinary research communications · 2023Review
- Changes in plasma alpha-1 acid glycoprotein following hemorrhagic trauma: Possible role in dose differences of ALM drug therapy in rat and pig resuscitation.Pharmacology research & perspectives · 2023Article
- Serum protein electrophoretic pattern in piglets during the early postnatal period.Scientific reports · 2021Article
- Serum Levels and Glycosylation Changes of Alpha-1-Acid Glycoprotein According to Severity of Breast Cancer in Korean Women.Journal of microbiology and biotechnology · 2020Article
- The Effect of Inactivated Mycobacterium Paratuberculosis Vaccine on the Response to a Heterologous Bacterial Challenge in Pigs.Frontiers in immunology · 2019Article
- Porcine Breed, Sex, and Production Stage Influence the Levels of Health Status Biomarkers in Saliva Samples.Frontiers in veterinary science · 2019Article
- Pharmacokinetic and Pharmacodynamic Considerations for Drugs Binding to Alpha-1-Acid Glycoprotein.Pharmaceutical research · 2018Review
- Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle.BMC veterinary research · 2018Article
- Regulation of cytokine gene expression by orosomucoid in neonatal swine adipose tissue.Journal of animal science and biotechnology · 2016Article
- Age- and Sex-Associated Effects on Acute-Phase Proteins in Göttingen Minipigs.Comparative medicine · 2015Article
- Article
- Functional network analysis of obese and lean Göttingen minipigs elucidates changes in oxidative and inflammatory networks in obese pigs.Pflugers Archiv : European journal of physiology · 2014Article
- Serum albumin and α-1 acid glycoprotein impede the killing of Schistosoma mansoni by the tyrosine kinase inhibitor Imatinib.International journal for parasitology. Drugs and drug resistance · 2014Article
- Fecal acute phase proteins in cats with chronic enteropathies.Journal of veterinary internal medicineArticle
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The serum protein α1-acid glycoprotein (AGP), also known as orosomucoid, is generally described as an archetypical positive acute phase protein. Here, porcine AGP was identified, purified and characterized from pooled pig serum. It was found to circulate as a single chain glycoprotein having an apparent molecular weight of 43 kDa by SDS-PAGE under reducing conditions, of which approximately 17 kDa were accounted for by N-bound oligosaccharides. Those data correspond well with the properties of the protein predicted from the single porcine AGP gene (ORM1, Q29014 (UniProt)), containing 5 putative glycosylation sites. A monoclonal antibody (MAb) was produced and shown to quantitatively and specifically react with all microheterogenous forms of pig AGP as analyzed by 2-D electrophoresis. This MAb was used to develop an immunoassay (ELISA) for quantification of AGP in pig serum samples. The adult serum concentrations of pig AGP were in the range of 1-3 mg/ml in a number of conventional pig breeds while it was lower in Göttingen and Ossabaw minipigs (in the 0.3 to 0.6 mg/ml range) and higher in young (2-5 days old) conventional pigs (mean: 6.6 mg/ml). Surprisingly, pig AGP was found to behave as a negative acute phase protein during a range of experimental infections and aseptic inflammation with significant decreases in serum concentration and in hepatic ORM1 expression during the acute phase response. To our knowledge this is the first description in any species of AGP being a negative acute phase protein.
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