ArticlePloS one2013
Light-sheet confined super-resolution using two-photon photoactivation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Wide-field two-photon random illumination microscopy (2P-RIM).Science advances · 2026Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- FastBiomedical optics express · 2020Article
- Between life and death: strategies to reduce phototoxicity in super-resolution microscopy.Journal of physics D: Applied physics · 2020Review
- Light-Sheet Microscopy in Neuroscience.Annual review of neuroscience · 2019Review
- Multimodal virtual histology of rabbit vocal folds by nonlinear microscopy and nano computed tomography.Biomedical optics express · 2019Article
- Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging.Communications biology · 2019Article
- Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections.Nature methods · 2018Article
- Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited].Optics express · 2018Review
- Adaptive optics stochastic optical reconstruction microscopy (AO-STORM) by particle swarm optimization.Biomedical optics express · 2017Article
- Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane.Chemical reviews · 2017Review
- New advances in probing cell-extracellular matrix interactions.Integrative biology : quantitative biosciences from nano to macro · 2017Review
- Assessing the imaging performance of light sheet microscopies in highly scattering tissues.Biomedical optics express · 2016Article
- Focusing super resolution on the cytoskeleton.F1000Research · 2016Review
- Light Sheet Fluorescence Microscopy (LSFM).Current protocols in cytometry · 2015Article
- Article
- Wide field intravital imaging by two-photon-excitation digital-scanned light-sheet microscopy (2p-DSLM) with a high-pulse energy laser.Biomedical optics express · 2014Article
- Eight years of single-molecule localization microscopy.Histochemistry and cell biology · 2014Review
- 3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy.Nature protocols · 2014Article
- Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting.Journal of the Royal Society, Interface · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light-sheet microscopy is a useful tool for performing biological investigations of thick samples and it has recently been demonstrated that it can also act as a suitable architecture for super-resolution imaging of thick biological samples by means of individual molecule localization. However, imaging in depth is still limited since it suffers from a reduction in image quality caused by scattering effects. This paper sets out to investigate the advantages of non-linear photoactivation implemented in a selective plane illumination configuration when imaging scattering samples. In particular, two-photon excitation is proven to improve imaging capabilities in terms of imaging depth and is expected to reduce light-sample interactions and sample photo-damage. Here, two-photon photoactivation is coupled to individual molecule localization methods based on light-sheet illumination (IML-SPIM), allowing super-resolution imaging of nuclear pH2AX in NB4 cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.