ArticleJournal of assisted reproduction and genetics2013
Retrieval and in vitro maturation of human oocytes from ovaries removed during surgery for endometrial carcinoma: a novel strategy for human oocyte research.
Article in Journal of assisted reproduction and genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Remodeling of maternal mRNA through poly(A) tail orchestrates human oocyte-to-embryo transition.Nature structural & molecular biology · 2023Article
- Functional substitution of zona pellucida with modified sodium hyaluronate gel in human embryos.Journal of assisted reproduction and genetics · 2022Article
- Oocyte collection and in vitro maturation after train transportation of human follicular fluid aspirated from resected non-stimulated ovaries of patients with endometrial adenocarcinoma.Reproductive medicine and biology · 2019Article
- In vitro maturation of oocytes from excised ovarian tissue in a patient with autoimmune ovarian insufficiency possibly associated with Epstein-Barr virus infection.Reproductive biology and endocrinology : RB&E · 2018Article
- In vitro maturation of human immature oocytes for fertility preservation and research material.Reproductive medicine and biology · 2017Review
- Vitrification of in vitro matured oocytes collected from surplus ovarian medulla tissue resulting from fertility preservation of ovarian cortex tissue.Journal of assisted reproduction and genetics · 2016Article
- Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets.Scientific reports · 2015Article
- In vitro maturation (IVM) of oocytes recovered from ovariectomy specimens in the laboratory: a promising "ex vivo" method of oocyte cryopreservation resulting in the first report of an ongoing pregnancy in Europe.Journal of assisted reproduction and genetics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo collect human oocytes from ovaries removed as part of surgical treatment for endometrial carcinoma, and to induce in vitro maturation of such oocytes to obtain material for research on human ovarian aging.
methods
designProspective clinical study.
settingUniversity Hospital. PATIENTS: Eight patients aged 35-44 years with a preoperative diagnosis of Stage I endometrial cancer agreed to participate in this project.
interventionsSurgically removed ovaries were punctured; oocytes were collected from follicular fluid and matured in vitro. Immunofluorescent detection of microtubules and DNA labeling were performed after in vitro maturation.
main outcome measuresNumber of oocytes collected and their in vitro maturation stage.
resultsIn total, 87 oocytes were collected, 11 of which had completed metaphase II. Of the oocytes collected, 75 % were from three patients in their 30s, while the remaining 25 % were from five patients in their 40s. Several stages of oocytes were collected and the detection of microtubule arrangement and chromatin in various stages using fluorescence was possible.
conclusionMaterial for research on human ovarian aging can be obtained from ovaries removed during surgery for endometrial cancer.
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