ArticleVirus genes2013
Isolation and molecular characterization of Newcastle disease virus genotypes II and VIId in Egypt between 2011 and 2012.
Article in Virus genes, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 107 citations in OpenAlex.
- Epidemiology of Newcastle disease in poultry in Africa: systematic review and meta-analysis.Tropical animal health and production · 2022Pooled it
- Prevalence, molecular characterization and phylogenetic analysis of Newcastle disease virus isolated from domestic poultry during 2019-2024 in Egypt.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Newcastle disease virus in Egyptian domestic poultry, 2019-2021: Molecular characterization, phylogenetic analysis, and coinfection with avian influenza A virus.Open veterinary journal · 2025Article
- A comprehensive pathological and molecular investigation of viral co-infections in ducks in Egypt.Frontiers in microbiology · 2025Article
- Protective effectiveness of two vaccination schemes against the prevalent Egyptian strain of Newcastle disease virus genotype VII.Open veterinary journal · 2024Article
- Article
- Active Surveillance and Genetic Characterization of Prevalent Velogenic Newcastle Disease and Highly Pathogenic Avian Influenza H5N8 Viruses Among Migratory Wild Birds in Southern Egypt During 2015-2018.Food and environmental virology · 2022Article
- Epidemiological surveillance of Newcastle disease virus in Egypt - a 6-year cohort study.Tropical animal health and production · 2022Article
- Article
- Pathogenesis of Velogenic Genotype VII.1.1 Newcastle Disease Virus Isolated from Chicken in Egypt via Different Inoculation Routes: Molecular, Histopathological, and Immunohistochemical Study.Animals : an open access journal from MDPI · 2021Article
- Phylogenetic studies of Newcastle disease virus isolated from poultry flocks in South Sulawesi Province, Indonesia, in 2019.Journal of advanced veterinary and animal research · 2021Article
- Molecular Characterization of Velogenic Newcastle Disease Virus (Sub-Genotype VII.1.1) from Wild Birds, with Assessment of Its Pathogenicity in Susceptible Chickens.Animals : an open access journal from MDPI · 2021Article
- AvianFrontiers in veterinary science · 2021Review
- Sequence analysis and pathogenicity of Avian Orthoavulavirus 1 strains isolated from poultry flocks during 2015-2019.BMC veterinary research · 2020Article
- Efficacy of inactivated velogenic Newcastle disease virus genotype VII vaccine in broiler chickens.Veterinary research forum : an international quarterly journal · 2020Article
- Molecular survey and interaction of common respiratory pathogens in chicken flocks (field perspective).Veterinary world · 2019Article
- Genotypes II and VIId-based inactivated Newcastle disease vaccine reduces virus shedding.Virusdisease · 2019Article
- Role of Pigeons in the Transmission of Avian Avulavirus (Newcastle Disease-Genotype VIId) to Chickens.Animals : an open access journal from MDPI · 2019Article
- Co-infections, genetic, and antigenic relatedness of avian influenza H5N8 and H5N1 viruses in domestic and wild birds in Egypt.Poultry science · 2019Article
- Combined H5ND inactivated vaccine protects chickens against challenge by different clades of highly pathogenic avian influenza viruses subtype H5 and virulent Newcastle disease virus.Veterinary world · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current study was conducted to isolate and characterize Newcastle disease virus (NDV) from recent outbreaks affecting poultry farms in Egypt between 2011 and 2012. Trachea, spleen, liver, proventriculus and caecal tonsils were collected from clinically infected NDV ten different vaccinated broiler farms in Fayoum, Behira and Giza Provinces. Inoculation of all the collected samples in 10-day-old embryonated chicken specific-pathogen-free eggs resulted in isolation of haemagglutinating agents in three samples. These haemagglutinating agents were confirmed as NDV by real-time reverse transcription polymerase chain reaction (rt RT-PCR) using matrix (M) gene-specific primers. The deduced amino acid sequences of the fusion protein revealed that one isolate possessed the motif (112)RRQKRF(117) at the cleavage site, indicating that this isolate is velogenic genotype, whereas the other two isolates carries the motif (112)GRQGRL(117) indicating they are lentogenic genotype. The phylogenetic analysis revealed that the velogenic genotype isolate clustered with published class II genotype VII sub genotype d NDVs and closely related to Middle East isolates, whereas the other two isolates clustered with published class II genotype II NDVs. The spread of velogenic genotype strain to Egypt via Middle Eastern countries is likely to be the source of infection.
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