ArticleMolecular microbiology2013
Post-transcriptional activation of a diguanylate cyclase by quorum sensing small RNAs promotes biofilm formation in Vibrio cholerae.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 50 citations in OpenAlex.
- Quorum sensing regulates a polysaccharide biosynthesis gene to control cell aggregation inISME communications · 2026Article
- A comparative genomic and phenotypic study ofMicrobiology (Reading, England) · 2024Article
- OpaR Exerts a Dynamic Control over c-di-GMP Homeostasis andMicrobiology spectrum · 2023Article
- Comprehensive insights into the metabolism characteristics of small RNA Qrr4 in Vibrio alginolyticus.Applied microbiology and biotechnology · 2023Article
- Small regulatory RNAs inmicroLife · 2023Review
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- The small RNA RsaF regulates the expression of secreted virulence factors in Staphylococcus aureus Newman.Journal of microbiology (Seoul, Korea) · 2021Article
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- Article
- The Multiple Regulatory Relationship Between RNA-Chaperone Hfq and the Second Messenger c-di-GMP.Frontiers in microbiology · 2021Review
- Impacts of Small RNAs and Their Chaperones on Bacterial Pathogenicity.Frontiers in cellular and infection microbiology · 2021Review
- Small noncoding RNA sRNA0426 is involved in regulating biofilm formation in Streptococcus mutans.MicrobiologyOpen · 2020Article
- Poly(A) polymerase is required for RyhB sRNA stability and function inRNA (New York, N.Y.) · 2018Article
- Article
- Article
- Alternative pathways for Escherichia coli biofilm formation revealed by sRNA overproduction.Molecular microbiology · 2017Article
- The ins and outs of cyclic di-GMP signaling in Vibrio cholerae.Current opinion in microbiology · 2017Review
- Article
- New insights from cluster analysis methods for RNA secondary structure prediction.Wiley interdisciplinary reviews. RNA · 2016Review
- Microbial Surface Colonization and Biofilm Development in Marine Environments.Microbiology and molecular biology reviews : MMBR · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Biofilms promote attachment of Vibrio cholerae in aquatic ecosystems and aid in transmission. Intracellular c-di-GMP levels that control biofilm development positively correlate with expression of Qrr sRNAs, which are transcribed when quorum sensing (QS) autoinducer levels are low. The Qrr sRNAs base-pair with and repress translation of hapR encoding the QS 'master regulator', hence increased c-di-GMP and biofilm development at low density were believed to be solely a consequence of Qrr/hapR pairing. We show that Qrr sRNAs also base-pair with and activate translation of the mRNA of a diguanylate cyclase (DGC), Vca0939; relieving an inhibitory structure in vca0939 that occludes the ribosome binding site. A nucleotide substitution in vca0939 disrupted sRNA/mRNA base-pairing and prevented vca0939 translation, while a compensating Qrr sRNA substitution restored pairing and Vca0939 levels. Qrr-dependent DGC activation led to c-di-GMP accumulation and biofilm development in V. cholerae. This represents the first description of (1) a DGC post-transcriptionally activated by direct pairing with an Hfq-dependent sRNA, and (2) control of a V. cholerae QS phenotype, independent of HapR. Thus, direct interactions of the same sRNAs with two mRNAs promote c-di-GMP-dependent biofilm formation by complementary mechanisms in V. cholerae; by negatively regulating HapR, and positively regulating the DGC Vca0939.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.