ArticlePloS one2013
Raman micro-spectroscopy can be used to investigate the developmental stage of the mouse oocyte.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Raman analysis of lipids in cells: Current applications and future prospects.Journal of pharmaceutical analysis · 2025Review
- Non-invasive applications of Raman spectroscopy in assisted reproduction.Frontiers in endocrinology · 2025Review
- Female-age-dependent changes in the lipid fingerprint of the mammalian oocytes.Human reproduction (Oxford, England) · 2024Article
- How great thou ART: biomechanical properties of oocytes and embryos as indicators of quality in assisted reproductive technologies.Frontiers in cell and developmental biology · 2024Review
- Biochemical changes in the cytoplasm of bovine oocytes during the in vitro maturation process: a Raman microscopy study.Veterinary research communications · 2022Article
- Evaluation of Laser Confocal Raman Spectroscopy as a Non-Invasive Method for Detecting Sperm DNA Contents.Frontiers in physiology · 2022Article
- Raman Spectroscopic Assessment of Myocardial Viability in Langendorff-Perfused Ischemic Rat Hearts.Acta histochemica et cytochemica · 2021Article
- Raman spectroscopic detection of carotenoids in cattle skin.RSC advances · 2020Article
- Surface enhanced Raman scattering artificial nose for high dimensionality fingerprinting.Nature communications · 2020Article
- Raman spectroscopy on live mouse early embryo while it continues to develop into blastocyst in vitro.Scientific reports · 2019Article
- Label-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes.Scientific reports · 2017Article
- Analysis of in vitro follicle development during the onset of premature ovarian insufficiency in a mouse model.Reproduction, fertility, and development · 2017Article
- Non-destructive monitoring of mouse embryo development and its qualitative evaluation at the molecular level using Raman spectroscopy.Scientific reports · 2017Article
- Raman-microscopy investigation of vitrification-induced structural damages in mature bovine oocytes.PloS one · 2017Article
- Evaluation of the impact of vitrification on the actin cytoskeleton of in vitro matured ovine oocytes by means of Raman microspectroscopy.Journal of assisted reproduction and genetics · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In recent years, the uptake of assisted reproductive techniques such as in vitro fertilisation has risen exponentially. However, there is much that is still not fully understood about the biochemical modifications that take place during the development and maturation of the oocyte. As such, it is essential to further the understanding of how oocyte manipulation during these procedures ultimately affects its developmental potential; yet, there are few methods currently available which are capable of providing a quantitative measure of oocyte quality. Raman spectroscopy enables investigation of the global biochemical profile of intact cells without the need for labelling. Here, Raman spectra were acquired from the ooplasm of mouse oocytes at various stages of development, from late pre-antral follicles, collected after in vitro maturation within their ovarian follicles and from unstimulated and stimulated ovulatory cycles. Using a combination of univariate and multivariate statistical methods, it was found that ooplasm lipid content could be used to discriminate between different stages of oocyte development. Furthermore, the spectral profiles of mature oocytes revealed that oocytes which have developed in vitro are protein-deficient when compared to in vivo grown oocytes. Finally, the ratio of two Raman peak intensities, namely 1605∶1447 cm⁻¹, used as a proxy for the protein-to-lipid ratio of the ooplasm, was shown to be indicative of the oocyte's quality. Together, results indicate that Raman spectroscopy may present an alternative analytical tool for investigating the biochemistry of oocyte developmental stage and quality.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.