ArticlePloS one2013
Detection of Streptococcus pneumoniae from Different Types of Nasopharyngeal Swabs in Children.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Streptococcus pneumoniae colonisation in children and adolescents with asthma: impact of the heptavalent pneumococcal conjugate vaccine and evaluation of potential effect of thirteen-valent pneumococcal conjugate vaccine.BMC infectious diseases · 2016Trial
- A Narrative Review of Remote Biospecimen Collection Product and Implementation Considerations for Sexual Health Clinical Research.AIDS and behavior · 2026Review
- Limited protection of pneumococcal vaccines against emergent Streptococcus pneumoniae serotype 14/ST876 strains.Infection · 2024Article
- Streptococcus pneumoniae and other bacterial nasopharyngeal colonization seven years post-introduction of 13-valent pneumococcal conjugate vaccine in South African children.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2023Article
- Development of a Microneedle Swab for Acquisition of Genomic DNA From Buccal Cells.Frontiers in bioengineering and biotechnology · 2022Article
- Molecular epidemiology of Staphylococcus aureus in African children from rural and urban communities with atopic dermatitis.BMC infectious diseases · 2021Article
- Covid-19 deaths in Africa: prospective systematic postmortem surveillance study.BMJ (Clinical research ed.) · 2021Article
- The influence of a swab type on the results of point-of-care tests.AMB Express · 2020Article
- Indoor air pollution and tobacco smoke exposure: impact on nasopharyngeal bacterial carriage in mothers and infants in an African birth cohort study.ERJ open research · 2019Article
- Evaluation of the SD Bioline Strep A Ultra Test in Relation With Number of Colony Forming Units and Color Intensity.Annals of laboratory medicine · 2019Article
- A comparison of cotton and flocked swabs for vaginal self-sample collection.International journal of women's health · 2018Article
- Incidence of Severe and Nonsevere Pertussis Among HIV-Exposed and -Unexposed Zambian Infants Through 14 Weeks of Age: Results From the Southern Africa Mother Infant Pertussis Study (SAMIPS), a Longitudinal Birth Cohort Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2016Article
- Respiratory microbes present in the nasopharynx of children hospitalised with suspected pulmonary tuberculosis in Cape Town, South Africa.BMC infectious diseases · 2016Article
- A comparison of flocked swabs and traditional swabs, using multiplex real-time PCR for detection of common gastroenteritis pathogens in Botswana.Diagnostic microbiology and infectious disease · 2016Article
- Pneumococcal colonization in older adults.Immunity & ageing : I & A · 2016Article
- Streptococcus pneumoniae oropharyngeal colonization in school-age children and adolescents with type 1 diabetes mellitus: Impact of the heptavalent pneumococcal conjugate vaccine.Human vaccines & immunotherapeutics · 2016Article
- Streptococcus pneumoniae pharyngeal colonization in school-age children and adolescents with cancer.Human vaccines & immunotherapeutics · 2016Article
- Laboratory Diagnosis of Pertussis.Clinical microbiology reviews · 2015Review
- Comparison of a Real-Time Multiplex PCR and Sequetyping Assay for Pneumococcal Serotyping.PloS one · 2015Article
- A comparison of swab types on sample adequacy, suspects comfort and provider preference in COVID-19.American journal of otolaryngologyArticle
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Authors and funding
5 authors.
Funding
Abstract
backgroundA better understanding of the epidemiology of nasopharyngeal carriage of Streptococcus pneumoniae is important to assess the impact of vaccination and the pathogenesis of pneumococcal disease. We compared the recovery of S. pneumoniae from nylon flocked, Dacron and rayon swabs.
methodsThe recovery of S. pneumoniae from mocked specimens using flocked, Dacron and rayon swabs were compared by culture. The yield from paired nasopharyngeal (NP) samples obtained from healthy children sampled with flocked and Dacron swabs was also determined using culture and lytA-targeted real-time polymerase chain reaction (qPCR).
resultsUsing mock specimen, the percentage recovery of S. pneumoniae ATCC 49619 (serotype 19F) strain from the flocked swabs was 100%, while it was 41% from Dacron swabs and 7% from rayon swabs. Similar results were observed for S. pneumoniae serotypes 1 and 5. S. pneumoniae was cultured from 18 of 42 (43%) paired NP samples from the healthy children (median age 8 [interquartile range (IQR) 5-16] months). The median number of colony-forming units (CFU) recovered from flocked swabs was two-fold higher (8.8×10(4) CFU/mL [IQR, 2.0×10(2) - 4.0×10(5) CFU/mL]) than Dacron swabs (3.7×10(4) CFU/mL [IQR, 4.0×10(2)-3.2×10(5) CFU/mL], p = 0.17). Using lytA-targeted qPCR from paired NP samples, the median copy number of S. pneumoniae detected from flocked swabs was significantly higher than from Dacron swabs (3.0×10(5) genome copies/mL [IQR, 1.3×10(2)-1.8×10(6)] vs. 9.3×10(4) genome copies/mL [IQR, 7.0×10(1)-1.1×10(6)]; p = 0.005).
conclusionFlocked swabs released more S. pneumoniae compared to both Dacron and rayon swabs from mock specimens. Similarly, higher bacterial loads were detected by qPCR from flocked swabs compared with Dacron swabs from healthy children.
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