ArticlePloS one2013
Modulation of Higher Order Chromatin Conformation in Mammalian Cell Nuclei Can Be Mediated by Polyamines and Divalent Cations.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 81 citations in OpenAlex.
- Proximity labeling at H3K9me3 reveals VRK-1 regulate global chromatin distribution inbioRxiv : the preprint server for biology · 2026Article
- Selective binding of divalent cations reshapes nucleosome mechanics and unlocks histone tail dynamics.Communications biology · 2026Article
- The shifting paradigm of chromatin structure: from the 30-nm chromatin fiber to liquid-like organization.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025Review
- Assessing the Noninferiority of the Spermidine Hyaluronate Complex Relative to 17β-Estradiol Treatment in the Ovariectomized Murine Model of Vulvovaginal Atrophy.Journal of menopausal medicine · 2025Article
- Probing chromatin condensation dynamics in live cells using interferometric scattering correlation spectroscopy.Communications biology · 2024Article
- Chromatin balances cell redox and energy homeostasis.Epigenetics & chromatin · 2023Review
- Evidence for low nanocompaction of heterochromatin in living embryonic stem cells.The EMBO journal · 2023Article
- FRET-FISH probes chromatin compaction at individual genomic loci in single cells.Nature communications · 2022Article
- Transgenic force sensors and software to measure force transmission across the mammalian nuclear envelope in vivo.Biology open · 2022Article
- Nucleosome destabilization by polyamines.Archives of biochemistry and biophysics · 2022Article
- Single-nucleosome imaging reveals steady-state motion of interphase chromatin in living human cells.Science advances · 2022Article
- Function and regulation of transcription factors during mitosis-to-G1 transition.Open biology · 2022Review
- Loops, topologically associating domains, compartments, and territories are elastic and robust to dramatic nuclear volume swelling.Scientific reports · 2022Article
- Stem Cell-Derived Extracellular Vesicles as Potential Therapeutic Approach for Acute Kidney Injury.Frontiers in immunology · 2022Review
- Nascent RNA scaffolds contribute to chromosome territory architecture and counter chromatin compaction.Molecular cell · 2021Article
- Physical Nature of Chromatin in the Nucleus.Cold Spring Harbor perspectives in biology · 2021Review
- ATR prevents CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Erythrocytes 3D genome organization in vertebrates.Scientific reports · 2021Article
- An Epigenetics-Based Hypothesis of Autoantigen Development in Systemic Lupus Erythematosus.Epigenomes · 2020Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The organisation of the large volume of mammalian genomic DNA within cell nuclei requires mechanisms to regulate chromatin compaction involving the reversible formation of higher order structures. The compaction state of chromatin varies between interphase and mitosis and is also subject to rapid and reversible change upon ATP depletion/repletion. In this study we have investigated mechanisms that may be involved in promoting the hyper-condensation of chromatin when ATP levels are depleted by treating cells with sodium azide and 2-deoxyglucose. Chromatin conformation was analysed in both live and permeabilised HeLa cells using FLIM-FRET, high resolution fluorescence microscopy and by electron spectroscopic imaging microscopy. We show that chromatin compaction following ATP depletion is not caused by loss of transcription activity and that it can occur at a similar level in both interphase and mitotic cells. Analysis of both live and permeabilised HeLa cells shows that chromatin conformation within nuclei is strongly influenced by the levels of divalent cations, including calcium and magnesium. While ATP depletion results in an increase in the level of unbound calcium, chromatin condensation still occurs even in the presence of a calcium chelator. Chromatin compaction is shown to be strongly affected by small changes in the levels of polyamines, including spermine and spermidine. The data are consistent with a model in which the increased intracellular pool of polyamines and divalent cations, resulting from depletion of ATP, bind to DNA and contribute to the large scale hyper-compaction of chromatin by a charge neutralisation mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.