ArticlePloS one2013
In vitro and in vivo infectious potential of coxiella burnetii: a study on Belgian livestock isolates.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- A systematic knowledge synthesis on the spatial dimensions of Q fever epidemics.Zoonoses and public health · 2019Pooled it
- Sources and culture methods for the isolation and cultivation ofFrontiers in microbiology · 2026Review
- Detection of abortifacient agents in domestic ruminants, with a specific focus onOpen research Europe · 2025Article
- QuilA® adjuvanted Coxevac® sustains Th1-CD8NPJ vaccines · 2023Article
- Phenotype ofPathogens (Basel, Switzerland) · 2022Article
- Q Fever-A Neglected Zoonosis.Microorganisms · 2022Review
- Correlating Genotyping Data ofPathogens (Basel, Switzerland) · 2021Article
- Article
- Phylogeography of Human and AnimalFrontiers in cellular and infection microbiology · 2020Article
- Extensive genome analysis of Coxiella burnetii reveals limited evolution within genomic groups.BMC genomics · 2019Article
- Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever.Frontiers in microbiology · 2019Article
- A Q Fever Outbreak with a High Rate of Abortions at a Dairy Goat Farm: Coxiella burnetii Shedding, Environmental Contamination, and Viability.Applied and environmental microbiology · 2018Article
- Management of Coxiella burnetii infection in livestock populations and the associated zoonotic risk: A consensus statement.Journal of veterinary internal medicine · 2018Article
- Comparison of genomes ofNew microbes and new infections · 2018Article
- Genome Plasticity and Polymorphisms in Critical Genes Correlate with Increased Virulence of Dutch Outbreak-RelatedFrontiers in microbiology · 2017Article
- From Q Fever to Coxiella burnetii Infection: a Paradigm Change.Clinical microbiology reviews · 2017Review
- Single Nucleotide Polymorphism Genotyping and Distribution of Coxiella burnetii Strains from Field Samples in Belgium.Applied and environmental microbiology · 2016Article
- Major differential gene regulation in Coxiella burnetii between in vivo and in vitro cultivation models.BMC genomics · 2015Article
- Article
- Serologic screening for 13 infectious agents in roe deer (Capreolus capreolus) in Flanders.Infection ecology & epidemiology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Q-fever is a zoonosis caused by the gram-negative obligate intracellular pathogen Coxiella burnetii. Since its discovery, and particularly following the recent outbreaks in the Netherlands, C. burnetii appeared as a clear public health concern. In the present study, the infectious potential displayed by goat and cattle isolates of C. burnetii was compared to a reference strain (Nine Mile) using both in vitro (human HeLa and bovine macrophage cells) and in vivo (BALB/c mice) models. The isolates had distant genomic profiles with one--the goat isolate--being identical to the predominant strain circulating in the Netherlands during the 2007-2010 outbreaks. Infective doses were established with ethidium monoazide-PCR for the first time here applied to C. burnetii. This method allowed for the preparation of reproducible and characterized inocula thanks to its capacity to discriminate between live and dead cells. Globally, the proliferative capacity of the Nine Mile strain in cell lines and mice was higher compared to the newly isolated field strains. In vitro, the bovine C. burnetii isolate multiplied faster in a bovine macrophage cell line, an observation tentatively explained by the preferential specificity of this strain for allogeneic host cells. In the BALB/c mouse model, however, the goat and bovine isolates multiplied at about the same rate indicating no peculiar hypervirulent behavior in this animal model.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.