ArticlePloS one2013
MOR is not enough: identification of novel mu-opioid receptor interacting proteins using traditional and modified membrane yeast two-hybrid screens.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Narrative review of the scientific and methodological explanations for 101 mechanisms of opioid tolerance.Pain reports · 2025Article
- Identification of TSPAN4 as Novel Histamine HBiomolecules · 2021Article
- In vivo mapping of a GPCR interactome using knockin mice.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Alternative splicing of the Wnt trafficking protein, Wntless and its effects on protein-protein interactions.BMC molecular and cell biology · 2019Article
- Identifying novel members of the Wntless interactome through genetic and candidate gene approaches.Brain research bulletin · 2018Article
- Molecular Pharmacology of δ-Opioid Receptors.Pharmacological reviews · 2016Review
- Early avoidance of a heroin-paired taste-cue and subsequent addiction-like behavior in rats.Brain research bulletin · 2016Article
- Low expression of D2R and Wntless correlates with high motivation for heroin.Behavioral neuroscience · 2015Article
- Heteromers of μ-δ opioid receptors: new pharmacology and novel therapeutic possibilities.British journal of pharmacology · 2015Review
- Effect of C-Terminal S-Palmitoylation on D2 Dopamine Receptor Trafficking and Stability.PloS one · 2015Article
- Bioorthogonal click chemistry to assay mu-opioid receptor palmitoylation using 15-hexadecynoic acid and immunoprecipitation.Analytical biochemistry · 2014Article
- Morphine-induced trafficking of a mu-opioid receptor interacting protein in rat locus coeruleus neurons.Progress in neuro-psychopharmacology & biological psychiatry · 2014Article
- Role of C-fibers in pain and morphine induced analgesia/hyperalgesia in rats.Iranian journal of neurology · 2014Article
- Annexin A2 traps mu-opioid receptors in recycling endosomes upon remifentanil-induced internalization.Neurobiology of pain (Cambridge, Mass.)Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The mu-opioid receptor (MOR) is the G-protein coupled receptor primarily responsible for mediating the analgesic and rewarding properties of opioid agonist drugs such as morphine, fentanyl, and heroin. We have utilized a combination of traditional and modified membrane yeast two-hybrid screening methods to identify a cohort of novel MOR interacting proteins (MORIPs). The interaction between the MOR and a subset of MORIPs was validated in pulldown, co-immunoprecipitation, and co-localization studies using HEK293 cells stably expressing the MOR as well as rodent brain. Additionally, a subset of MORIPs was found capable of interaction with the delta and kappa opioid receptors, suggesting that they may represent general opioid receptor interacting proteins (ORIPS). Expression of several MORIPs was altered in specific mouse brain regions after chronic treatment with morphine, suggesting that these proteins may play a role in response to opioid agonist drugs. Based on the known function of these newly identified MORIPs, the interactions forming the MOR signalplex are hypothesized to be important for MOR signaling and intracellular trafficking. Understanding the molecular complexity of MOR/MORIP interactions provides a conceptual framework for defining the cellular mechanisms of MOR signaling in brain and may be critical for determining the physiological basis of opioid tolerance and addiction.
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