ArticlePloS one2013
Identification of differentially expressed genes of Trichinella spiralis larvae after exposure to host intestine milieu.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Effects of Trichinella spiralis and its serine protease inhibitors on intestinal mucosal barrier function.Veterinary research · 2025Article
- Early diagnosis ofVeterinary world · 2024Article
- Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis.Parasites & vectors · 2024Article
- Molecular characterization of a novel serine proteinase from Trichinella spiralis and its participation in larval invasion of gut epithelium.PLoS neglected tropical diseases · 2023Article
- Oral immunization of mice with recombinant Lactobacillus plantarum expressing a Trichinella spiralis galectin induces an immune protection against larval challenge.Parasites & vectors · 2022Article
- Characterization of a novel pyruvate kinase from Trichinella spiralis and its participation in sugar metabolism, larval molting and development.PLoS neglected tropical diseases · 2022Article
- Proteases secreted by Trichinella spiralis intestinal infective larvae damage the junctions of the intestinal epithelial cell monolayer and mediate larval invasion.Veterinary research · 2022Article
- Disruption of Epithelial Barrier of Caco-2 Cell Monolayers by Excretory Secretory Products ofFrontiers in microbiology · 2021Article
- Characterization of a Novel Glutamine Synthetase FromFrontiers in cell and developmental biology · 2021Article
- Characterization of a Trichinella spiralis aminopeptidase and its participation in invasion, development and fecundity.Veterinary research · 2020Article
- Comparative expression profiles of host circulating miRNAs in response to Trichinella spiralis infection.Veterinary research · 2020Article
- Molecular characterization of a Trichinella spiralis elastase-1 and its potential as a diagnostic antigen for trichinellosis.Parasites & vectors · 2020Article
- In vitro silencing of a serine protease inhibitor suppresses Trichinella spiralis invasion, development, and fecundity.Parasitology research · 2019Article
- Regulation of host immune cells and cytokine production induced by Trichinella spiralis infection.Parasite (Paris, France) · 2019Article
- Oral vaccination with Trichinella spiralis DNase II DNA vaccine delivered by attenuated Salmonella induces a protective immunity in BALB/c mice.Veterinary research · 2018Article
- Protective immunity against Trichinella spiralis in mice elicited by oral vaccination with attenuated Salmonella-delivered TsSP1.2 DNA.Veterinary research · 2018Article
- Molecular characterization of a putative serine protease from Trichinella spiralis and its elicited immune protection.Veterinary research · 2018Article
- Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool.PLoS neglected tropical diseases · 2018Article
- Characterization of TwoFrontiers in microbiology · 2018Article
- Cloning and expression of a Trichinella spiralis putative glutathione S-transferase and its elicited protective immunity against challenge infections.Parasites & vectors · 2017Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although it has been known for many years that T. spiralis muscle larvae (ML) can not invade intestinal epithelial cells unless they are exposed to the intestinal milieu and activated into intestinal infective larvae (IIL), which genes in IIL are involved in the process of invasion is still unknown. In this study, suppression subtractive hybridization (SSH) was performed to identify differentially expressed genes between IIL and ML. SSH library was constructed using cDNA generated from IIL as the 'tester'. About 110 positive clones were randomly selected from the library and sequenced, of which 33 T. spiralis genes were identified. Thirty encoded proteins were annotated according to Gene Ontology Annotation in terms of molecular function, biological process, and cellular localization. Out of 30 annotated proteins, 16 proteins (53.3%) had binding activity and 12 proteins (40.0%) had catalytic activity. The results of real-time PCR showed that the expression of nine genes (Ts7, Ndr family protein; Ts8, serine/threonine-protein kinase polo; Ts11, proteasome subunit beta type-7; Ts17, nudix hydrolase; Ts19, ovochymase-1; Ts22, fibronectin type III domain protein; Ts23, muscle cell intermediate filament protein OV71; Ts26, neutral and basic amino acid transport protein rBAT and Ts33, FACT complex subunit SPT16) from 33 T. spiralis genes in IIL were up-regulated compared with that of ML. The present study provide a group of the potential invasion-related candidate genes and will be helpful for further studies of mechanisms by which T. spiralis infective larvae recognize and invade the intestinal epithelial cells.
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