ArticlePloS one2013
Expression and significance of neuroligins in myenteric cells of Cajal in Hirschsprung's disease.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Effect of Neuroligin1 and Neurexin1 on the Colonic Motility in a Mouse Model of Neuronal Intestinal Dysplasia.Gastroenterology research and practice · 2020Article
- Gastrointestinal dysfunction in patients and mice expressing the autism-associated R451C mutation in neuroligin-3.Autism research : official journal of the International Society for Autism Research · 2019Article
- Hirschsprung's disease - Postsurgical intestinal dysmotility.Revista paulista de pediatria : orgao oficial da Sociedade de Pediatria de Sao Paulo · 2016Article
- Video Imaging and Spatiotemporal Maps to Analyze Gastrointestinal Motility in Mice.Journal of visualized experiments : JoVE · 2016Article
- Abundance and significance of neuroligin-1 and glutamate in Hirschsprung's disease.World journal of gastroenterology · 2015Article
- The down-regulation of neuroligin-2 and the correlative clinical significance of serum GABA over-expression in Hirschsprung's disease.Neurochemical research · 2014Article
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7 authors.
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Abstract
purposeThe aim of this study was to investigate the expression and significance of neuroligins in myenteric cells of Cajal (ICC-MY) in Hirschsprung's disease (HSCR).
methodsLongitudinal muscle with adherent myenteric plexus (LMMP) from surgical excision waste colon of HSCR children were prepared by peeling off the mucous layer, sub-mucosal layer and circular muscle. Neuroligins, c-Kit (c-Kit-immunoreactivity representing ICC) and their relationship were assessed by double labeling immunofluorescence staining. ICC-MY were dissociated and cultured from LMMP by enzymolysis method, and were purified and analyzed using a combination of magnetic-activated cell sorting (MACS) and flow cytometry (FCM). Western-blot analysis was applied to compare and evaluate the expression levels of neuroligins in ICC-MY which were dissociated from different segments of HSCR (ganglionic colonic segment, transitional colonic segment and aganglionic colonic segment).
resultsNeuroligins and c-Kit were expressed on the same cells (ICC-MY); ICC-MY were dissociated, cultured and purified. For HSCR, neuroligins were expressed significantly in ICC-MY from ganglionic colonic segments, moderately in those from transitional colonic segments and down-regulated significantly in those from aganglionic colonic segments.
conclusionsNeuroligins were expressed in ICC-MY of human beings, and the expression varies from different segments of HSCR. This abnormal expression might play an important role in the pathogenesis of this disease through affecting the synaptic function of ICC-MY.
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