ArticlePloS one2013
Strategies for Wheat Stripe Rust Pathogenicity Identified by Transcriptome Sequencing.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 123 citations in OpenAlex.
- RNA-Seq Profiling Identifies Temperature-Responsive Genes in Germinating Urediniospores ofJournal of fungi (Basel, Switzerland) · 2026Article
- Virulence gains in the Puccinia striiformis f. sp. tritici PstS10 lineage correlate with expression polymorphism in a candidate Avr effector.Communications biology · 2026Article
- Sugars, Lipids and More: New Insights Into Plant Carbon Sources During Plant-Microbe Interactions.Plant, cell & environment · 2025Review
- Transcriptome, miRNA, and degradome sequencing reveal the leaf stripe (Pyrenophora graminea) resistance genes in Tibetan hulless barley.BMC plant biology · 2025Article
- Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes.International journal of molecular sciences · 2024Article
- Article
- High-throughput RNA sequencing reveals differences between the transcriptomes of the five spore forms of Puccinia striiformis f. sp. tritici, the wheat stripe rust pathogen.Stress biology · 2023Article
- The Biological Roles ofBiomolecules · 2023Review
- Molecular Mechanisms of the Co-Evolution of Wheat and Rust Pathogens.Plants (Basel, Switzerland) · 2023Review
- Transcriptome Comparison between Two Strains ofJournal of fungi (Basel, Switzerland) · 2022Article
- Current Status and Future Perspectives of Genomics Research in the Rust Fungi.International journal of molecular sciences · 2022Review
- Epigenetics of wheat-rust interaction: an update.Planta · 2022Review
- Review
- Review
- The haustorium: The root of biotrophic fungal pathogens.Frontiers in plant science · 2022Review
- Effectors ofInternational journal of molecular sciences · 2021Article
- Rust expression browser: an open source database for simultaneous analysis of host and pathogen gene expression profiles with expVIP.BMC genomics · 2021Article
- Article
- In-depth secretome analysis of Puccinia striiformis f. sp. tritici in infected wheat uncovers effector functions.Bioscience reports · 2020Article
- A holistic view on plant effector-triggered immunity presented as an iceberg model.Cellular and molecular life sciences : CMLS · 2020Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stripe rust caused by the fungus Puccinia striiformis f.sp. tritici (Pst) is a major constraint to wheat production worldwide. The molecular events that underlie Pst pathogenicity are largely unknown. Like all rusts, Pst creates a specialized cellular structure within host cells called the haustorium to obtain nutrients from wheat, and to secrete pathogenicity factors called effector proteins. We purified Pst haustoria and used next-generation sequencing platforms to assemble the haustorial transcriptome as well as the transcriptome of germinated spores. 12,282 transcripts were assembled from 454-pyrosequencing data and used as reference for digital gene expression analysis to compare the germinated uredinospores and haustoria transcriptomes based on Illumina RNAseq data. More than 400 genes encoding secreted proteins which constitute candidate effectors were identified from the haustorial transcriptome, with two thirds of these up-regulated in this tissue compared to germinated spores. RT-PCR analysis confirmed the expression patterns of 94 effector candidates. The analysis also revealed that spores rely mainly on stored energy reserves for growth and development, while haustoria take up host nutrients for massive energy production for biosynthetic pathways and the ultimate production of spores. Together, these studies substantially increase our knowledge of potential Pst effectors and provide new insights into the pathogenic strategies of this important organism.
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