ArticlePloS one2013
Cell lineage identification and stem cell culture in a porcine model for the study of intestinal epithelial regeneration.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
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Who cites it
94 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis identifies the effect of dietary multi-enzyme supplementation on gut health of pigs.Scientific reports · 2021Pooled it
- Animal organoids as models for integrated One Health research.One health (Amsterdam, Netherlands) · 2026Review
- A Pilot Study for Establishing DSS-Induced Colitis Model in Minipigs.Tissue engineering and regenerative medicine · 2026Article
- Influence of the drone brood homogenate on the gut integrity and cellular immunity: A pilot study on pigs.Veterinarni medicina · 2026Article
- A Shared Framework for Gastrointestinal Organoid Research: It's Time.Cellular and molecular gastroenterology and hepatology · 2026Article
- Age-dependent functional and transcriptional differences in intestinal mesenchymal stromal cells during early postnatal development in piglets.Frontiers in cell and developmental biology · 2026Article
- Functional characteristics of a pig 2D intestinal organoid model as anJournal of animal science and technology · 2026Article
- Relevance of porcine intestinal organoids as a surrogate for animal experimentation: application to the investigation of host-virus interactions during porcine coronavirus infection.Veterinary research · 2025Article
- Organoids in parasitology: a game-changer for studying host-nematode interactions.Parasitology · 2025Review
- Porcine intestinal organoids cultured in an organ-on-a-chip microphysiological system.Biochemistry and biophysics reports · 2025Article
- Growth performance and total tract digestibility of nutrients for weanling pigs are improved by an exogenous xylanase and a stimbiotic regardless of maternal xylanase consumption.Journal of animal science and biotechnology · 2025Article
- Development of Sheep Intestinal Organoids for Studying Deoxynivalenol-Induced Toxicity.International journal of molecular sciences · 2025Article
- Development and characterization of a 2D porcine colonic organoid model for studying intestinal physiology and barrier function.PloS one · 2025Article
- Article
- Initial pig developmental stage influences intestinal organoid growth but not cellular composition.Animal models and experimental medicine · 2024Article
- Comparative evaluation of the modulatory role of 1,25-dihydroxy-vitamin DJournal of animal science and biotechnology · 2024Article
- Culture media and format alter cellular composition and barrier integrity of porcine colonoid-derived monolayers.Tissue barriers · 2024Article
- Comparison of the effects of normothermic machine perfusion and cold storage preservation on porcine intestinal allograft regenerative potential and viability.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2024Article
- A matter of differentiation: equine enteroids as a model for the in vivo intestinal epithelium.Veterinary research · 2024Article
- Adult Animal Stem Cell-Derived Organoids in Biomedical Research and the One Health Paradigm.International journal of molecular sciences · 2024Review
34 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Significant advances in intestinal stem cell biology have been made in murine models; however, anatomical and physiological differences between mice and humans limit mice as a translational model for stem cell based research. The pig has been an effective translational model, and represents a candidate species to study intestinal epithelial stem cell (IESC) driven regeneration. The lack of validated reagents and epithelial culture methods is an obstacle to investigating IESC driven regeneration in a pig model. In this study, antibodies against Epithelial Adhesion Molecule 1 (EpCAM) and Villin marked cells of epithelial origin. Antibodies against Proliferative Cell Nuclear Antigen (PCNA), Minichromosome Maintenance Complex 2 (MCM2), Bromodeoxyuridine (BrdU) and phosphorylated Histone H3 (pH3) distinguished proliferating cells at various stages of the cell cycle. SOX9, localized to the stem/progenitor cells zone, while HOPX was restricted to the +4/'reserve' stem cell zone. Immunostaining also identified major differentiated lineages. Goblet cells were identified by Mucin 2 (MUC2); enteroendocrine cells by Chromogranin A (CGA), Gastrin and Somatostatin; and absorptive enterocytes by carbonic anhydrase II (CAII) and sucrase isomaltase (SIM). Transmission electron microscopy demonstrated morphologic and sub-cellular characteristics of stem cell and differentiated intestinal epithelial cell types. Quantitative PCR gene expression analysis enabled identification of stem/progenitor cells, post mitotic cell lineages, and important growth and differentiation pathways. Additionally, a method for long-term culture of porcine crypts was developed. Biomarker characterization and development of IESC culture in the porcine model represents a foundation for translational studies of IESC-driven regeneration of the intestinal epithelium in physiology and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.