ArticlePloS one2013
Increase of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of Crohn's disease with a multidisciplinary platform.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Recent advances in characterizing the gastrointestinal microbiome in Crohn's disease: a systematic review.Inflammatory bowel diseases · 2015Pooled it
- Trial
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- The Role of Proteolysis in Amyloidosis.International journal of molecular sciences · 2022Review
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- Systematic Review: The Gut Microbiome and Its Potential Clinical Application in Inflammatory Bowel Disease.Microorganisms · 2021Review
- A semi-tryptic peptide centric metaproteomic mining approach and its potential utility in capturing signatures of gut microbial proteolysis.Microbiome · 2021Article
- A serine protease secreted from Bacillus subtilis cleaves human plasma transthyretin to generate an amyloidogenic fragment.Communications biology · 2020Article
- Fecal Serine Protease Profiling in Inflammatory Bowel Diseases.Frontiers in cellular and infection microbiology · 2020Article
- Plasma amino acid profiles in dogs with inflammatory bowel disease.Journal of veterinary internal medicine · 2019Article
- Increased Pancreatic Protease Activity in Response to Antibiotics Impairs Gut Barrier and Triggers Colitis.Cellular and molecular gastroenterology and hepatology · 2018Article
- Cross sectional evaluation of the gut-microbiome metabolome axis in an Italian cohort of IBD patients.Scientific reports · 2017Article
- Unconjugated bilirubin ameliorates the inflammation and digestive protease increase in TNBS-induced colitis.Molecular medicine reports · 2017Article
- Substrate Profiling and High Resolution Co-complex Crystal Structure of a Secreted C11 Protease Conserved across Commensal Bacteria.ACS chemical biology · 2017Article
- Intestinal microbiome in children with severe and complicated acute viral gastroenteritis.Scientific reports · 2017Article
- Role of antibiotics for treatment of inflammatory bowel disease.World journal of gastroenterology · 2016Review
- Microbiota and host determinants of behavioural phenotype in maternally separated mice.Nature communications · 2015Article
- Manipulating the gut microbiota to maintain health and treat disease.Microbial ecology in health and disease · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo investigate-by molecular, classical and functional methods-the microbiota in biopsies and faeces from patients with active Crohn's disease (CD) and controls.
designThe microbiota in biopsies was investigated utilizing a novel molecular method and classical cultivation technology. Faecal samples were investigated by classical technology and four functional methods, reflecting alterations in short chain fatty acids pattern, conversion of cholesterol and bilirubin and inactivation of trypsin.
resultsBy molecular methods we found more than 92% similarity in the microbiota on the biopsies from the two groups. However, 4.6% of microbes found in controls were lacking in CD patients. Furthermore, NotI representation libraries demonstrate two different clusters representing CD patients and controls, respectively. Utilizing conventional technology, Bacteroides (alt. Parabacteroides) was less frequently detected in the biopsies from CD patients than from controls. A similar reduction in the number of Bacteroides was found in faecal samples. Bacteroides is the only group of bacteria known to be able to inactivate pancreatic trypsin. Faecal tryptic activity was high in CD patients, and inversely correlated to the levels of Bacteroides.
conclusionsCD patients have compositional and functional alterations in their intestinal microbiota, in line with the global description hypothesis rather than the candidate microorganism theory. The most striking functional difference was high amount of faecal tryptic activity in CD patients, inversely correlated to the levels of Bacteroides in faeces.
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