ArticleEvidence-based complementary and alternative medicine : eCAM2013
Chemical Composition and Anti-Inflammatory Effect of Ethanolic Extract of Brazilian Green Propolis on Activated J774A.1 Macrophages.
Article in Evidence-based complementary and alternative medicine : eCAM, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 101 citations in OpenAlex.
- Propolis supplementation on inflammatory and oxidative stress biomarkers in adults: a systematic review and meta-analysis of randomized controlled trials.Frontiers in nutrition · 2025Pooled it
- The Influence of Ethanolic Extract of Brazilian Green Propolis Gel on Hygiene and Oral Microbiota in Patients after Mandible Fractures.BioMed research international · 2016Trial
- Immunomodulatory Effects of Propolis on Endothelial Cytokine Release.Molecules (Basel, Switzerland) · 2026Article
- Seasonal Variation of a New Brazilian Greenish-Brown Propolis Type: Chemical Composition and Antioxidant, Antimicrobial, and Antileishmanial Activities.Molecules (Basel, Switzerland) · 2026Article
- Assessment of the Polyphenolic Profile and Beneficial Effects of Red and Green Propolis in Skin Inflammatory Conditions and Oxidative Stress.Biomedicines · 2025Article
- The Effect of Ethanolic Extract of Brazilian Green Propolis and Artepillin C on Cytokine Secretion by Stage IV Glioma Cells Under Hypoxic and Normoxic Conditions.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Effects of propolis extract supplementation in breeder and broiler diets and it's in ovo injection on immune status, blood parameters, vaccine-antibody response and intestinal microflora of broiler chick.Tropical animal health and production · 2025Article
- Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms.Pathogens (Basel, Switzerland) · 2023Article
- Evaluation of Chemical Composition, Sun Protection Factor and Antioxidant Activity of Lithuanian Propolis and Its Plant Precursors.Plants (Basel, Switzerland) · 2022Article
- Bioinductive and anti-inflammatory properties of Propolis and Biodentine on SHED.The Saudi dental journal · 2022Article
- Enhancement of the Antioxidant Capacity of Thyme and Chestnut Honey by Addition of Bee Products.Foods (Basel, Switzerland) · 2022Article
- Does propolis have any effect on rheumatoid arthritis? A review study.Food science & nutrition · 2022Review
- Synergistic Effect of Polyphenol-Rich Complex of Plant and Green Propolis Extracts with Antibiotics against Respiratory Infections Causing Bacteria.Antibiotics (Basel, Switzerland) · 2022Article
- Effects of Standardized Brazilian Green Propolis Extract (EPP-AF®) on Inflammation in Haemodialysis Patients: A Clinical Trial.International journal of nephrology · 2022Article
- The Effect of Chitosan Type on Biological and Physicochemical Properties of Films with Propolis Extract.Polymers · 2021Article
- A New Approach to Atopic Dermatitis Control with Low-Concentration Propolis-Loaded Cold Cream.Pharmaceutics · 2021Article
- Antiviral, Antibacterial, Antifungal, and Antiparasitic Properties of Propolis: A Review.Foods (Basel, Switzerland) · 2021Review
- Attenuation of carrageenan-induced hind paw edema and plasma TNF-α level by Philippine stingless bee (Tetragonula biroi Friese) propolis.Experimental animals · 2021Article
- Potential Role of Propolis in the Prevention and Treatment of Metabolic Diseases.Plants (Basel, Switzerland) · 2021Review
- Green propolis extract attenuates acute kidney injury and lung injury in a rat model of sepsis.Scientific reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to investigate the chemical composition and anti-inflammatory effect of ethanolic extract of Brazilian green propolis (EEP-B) on LPS + IFN- γ or PMA stimulated J774A.1 macrophages. The identification and quantification of phenolic compounds in green propolis extract were performed using HPLC-DAD and UPLC-Q-TOF-MS methods. The cell viability was evaluated by MTT and LDH assays. The radical scavenging ability was determined using DPPH(•) and ABTS(•+). ROS and RNS generation was analyzed by chemiluminescence. NO concentration was detected by the Griess reaction. The release of various cytokines by activated J774A.1 cells was measured in the culture supernatants using a multiplex bead array system based on xMAP technology. Artepillin C, kaempferide, and their derivatives were the main phenolics found in green propolis. At the tested concentrations, the EEP-B did not decrease the cell viability and did not cause the cytotoxicity. EEP-B exerted strong antioxidant activity and significantly inhibited the production of ROS, RNS, NO, cytokine IL-1 α , IL-1 β , IL-4, IL-6, IL-12p40, IL-13, TNF- α , G-CSF, GM-CSF, MCP-1, MIP-1 α , MIP-1 β , and RANTES in stimulated J774A.1 macrophages. Our findings provide new insights for understanding the anti-inflammatory mechanism of action of Brazilian green propolis extract and support its application in complementary and alternative medicine.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.