ArticleCell cycle (Georgetown, Tex.)2013
Metabolome and metaboproteome remodeling in nuclear reprogramming.
Article in Cell cycle (Georgetown, Tex.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Species-specific metabolic reprogramming in human and mouse microglia during inflammatory pathway induction.Nature communications · 2023Article
- Crotonylation of GAPDH regulates human embryonic stem cell endodermal lineage differentiation and metabolic switch.Stem cell research & therapy · 2023Article
- Diversity of respiratory parameters and metabolic adaptation to low oxygen tension in mesenchymal stromal cells.Metabolism open · 2022Article
- Extracellular Flux Analysis of Mitochondrial Function in Pluripotent Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Metabolic Coordination of Pericyte Phenotypes: Therapeutic Implications.Frontiers in cell and developmental biology · 2020Review
- In Vitro Induction of Pluripotency from Equine Fibroblasts in 20% or 5% Oxygen.Stem cells international · 2020Article
- Impaired cardiac performance, protein synthesis, and mitochondrial function in tumor-bearing mice.PloS one · 2019Article
- Metabolism Is a Key Regulator of Induced Pluripotent Stem Cell Reprogramming.Stem cells international · 2019Review
- 1α,25-dihydroxyvitamin DBiochemical and biophysical research communications · 2018Article
- Physiological oxygen culture reveals retention of metabolic memory in human induced pluripotent stem cells.PloS one · 2018Article
- Temporal depolarization of mitochondria during M phase.Scientific reports · 2017Article
- Glycolytic Metabolism Plays a Functional Role in Regulating Human Pluripotent Stem Cell State.Cell stem cell · 2016Article
- Article
- 1α,25-Dihydroxyvitamin D3 Regulates Mitochondrial Oxygen Consumption and Dynamics in Human Skeletal Muscle Cells.The Journal of biological chemistry · 2016Article
- GLUT3 and PKM2 regulate OCT4 expression and support the hypoxic culture of human embryonic stem cells.Scientific reports · 2015Article
- Hepatocyte selection medium eliminating induced pluripotent stem cells among primary human hepatocytes.World journal of methodology · 2015Article
- Development of a protein marker panel for characterization of human induced pluripotent stem cells (hiPSCs) using global quantitative proteome analysis.Stem cell research · 2015Article
- miR-290/371-Mbd2-Myc circuit regulates glycolytic metabolism to promote pluripotency.The EMBO journal · 2015Article
- Metabolic control of cancer cell stemness: Lessons from iPS cells.Cell cycle (Georgetown, Tex.) · 2015Review
- Metabostemness: a new cancer hallmark.Frontiers in oncology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Nuclear reprogramming resets differentiated tissue to generate induced pluripotent stem (iPS) cells. While genomic attributes underlying reacquisition of the embryonic-like state have been delineated, less is known regarding the metabolic dynamics underscoring induction of pluripotency. Metabolomic profiling of fibroblasts vs. iPS cells demonstrated nuclear reprogramming-associated induction of glycolysis, realized through augmented utilization of glucose and accumulation of lactate. Real-time assessment unmasked downregulated mitochondrial reserve capacity and ATP turnover correlating with pluripotent induction. Reduction in oxygen consumption and acceleration of extracellular acidification rates represent high-throughput markers of the transition from oxidative to glycolytic metabolism, characterizing stemness acquisition. The bioenergetic transition was supported by proteome remodeling, whereby 441 proteins were altered between fibroblasts and derived iPS cells. Systems analysis revealed overrepresented canonical pathways and interactome-associated biological processes predicting differential metabolic behavior in response to reprogramming stimuli, including upregulation of glycolysis, purine, arginine, proline, ribonucleoside and ribonucleotide metabolism, and biopolymer and macromolecular catabolism, with concomitant downregulation of oxidative phosphorylation, phosphate metabolism regulation, and precursor biosynthesis processes, prioritizing the impact of energy metabolism within the hierarchy of nuclear reprogramming. Thus, metabolome and metaboproteome remodeling is integral for induction of pluripotency, expanding on the genetic and epigenetic requirements for cell fate manipulation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.