ArticleStem cell research & therapy2013
Adipogenesis of adipose-derived stem cells may be regulated via the cytoskeleton at physiological oxygen levels in vitro.
Article in Stem cell research & therapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 56 citations in OpenAlex.
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- Directed Cell Self-Assembly to Form Tendon and Muscle Models for Studying Early Stages of Musculoskeletal Tissue Formation.Journal of biomechanical engineering · 2026Article
- Development of a sustainable strategy for cultured fat production based on serum-free 3D culture of bovine adipose stem cells.Scientific reports · 2025Article
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- Mechanical Cell Reprogramming on Tissue-Mimicking Hydrogels for Cancer Cell Transdifferentiation.Research (Washington, D.C.) · 2025Article
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- Super-enhancer RNA mNature genetics · 2023Article
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- Cytochalasin B Modulates Nanomechanical Patterning and Fate in Human Adipose-Derived Stem Cells.Cells · 2022Article
- Impact of Microenvironmental Changes during Degeneration on Intervertebral Disc Progenitor Cells: A Comparison with Mesenchymal Stem Cells.Bioengineering (Basel, Switzerland) · 2022Review
- The effects of 3D culture on the expansion and maintenance of nucleus pulposus progenitor cell multipotency.JOR spine · 2021Article
- Adipocytes fail to maintain cellular identity during obesity due to reduced PPARγ activity and elevated TGFβ-SMAD signaling.Molecular metabolism · 2020Article
- Molecular Mechanisms of Adipogenesis: The Anti-adipogenic Role of AMP-Activated Protein Kinase.Frontiers in molecular biosciences · 2020Review
- Actomyosin-Mediated Tension Orchestrates Uncoupled Respiration in Adipose Tissues.Cell metabolism · 2018Article
- Platelet Lysate Activates Human Subcutaneous Adipose Tissue Cells by Promoting Cell Proliferation and Their Paracrine Activity Toward Epidermal Keratinocytes.Frontiers in bioengineering and biotechnology · 2018Article
- A Review of Cell-Based Strategies for Soft Tissue Reconstruction.Tissue engineering. Part B, Reviews · 2017Review
- A genome-wide analysis of the gene expression profiles and alternative splicing events during the hypoxia-regulated osteogenic differentiation of human cartilage endplate-derived stem cells.Molecular medicine reports · 2017Article
- Effect of hypoxia on human adipose-derived mesenchymal stem cells and its potential clinical applications.Cellular and molecular life sciences : CMLS · 2017Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
introductionObesity, which is excessive expansion of white adipose tissue, is a major risk factor for several serious health issues, including diabetes, cardiovascular disease and cancer. Efforts to combat obesity and related diseases require understanding the basic biology of adipogenesis. However, in vitro studies do not result in lipid composition and morphology that are typically seen in vivo, likely because the in vitro conditions are not truly representative of in vivo adipose tissue formation. In vitro, low oxygen tension and cytoskeletal tension have been shown to independently regulate adipogenesis, but in vivo, these two factors simultaneously influence differentiation.
methodsThe purpose of our study was to examine the influence of physiological oxygen tension on cytoskeletal tension-mediated adipogenesis. Adipose-derived stem cells (ASCs) were differentiated under both ambient (20%) and physiological (5%) oxygen conditions and treated with cytoskeletal inhibitors, cytochalasin D or blebbistatin. Adipogenesis was assessed on the basis of gene expression and adipocyte metabolic function.
resultsAdipose tissue metabolic markers (glycerol-3-phosphate dehydrogenase (GPDH) and triglycerides) were significantly down-regulated by physiological oxygen levels. Reducing cytoskeletal tension through the use of chemical inhibitors, either cytochalasin D or blebbistatin, resulted in an up-regulation of adipogenic gene expression (peroxisome proliferator-activated receptor γ (PPARγ), lipoprotein lipase (LPL) and fatty acid binding protein 4 (FABP4)) and metabolic markers, regardless of oxygen levels. Cytochalasin D and blebbistatin treatment altered cytoskeletal organization and associated tension via different mechanisms; however, both conditions had similar effects on adipogenesis, suggesting that physiological oxygen-mediated regulation of adipogenesis in ASCs is modulated, in part, by cytoskeletal tension.
conclusionsThese results demonstrated that interactions between the cytoskeleton and oxygen tension influence adipogenic differentiation of ASCs.
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