ArticleThe Journal of biological chemistry2013
Active plasma membrane P-type H+-ATPase reconstituted into nanodiscs is a monomer.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Isolation and characterization of plant plasma membrane P-type HScientific reports · 2026Article
- Functional reconstitution of plant plasma membrane HScientific reports · 2025Article
- Molecular tipping points in plant cell surface HQuantitative plant biology · 2025Review
- Uncovering diffusive states of the yeast membrane protein, Pma1, and how labeling method can change diffusive behavior.The European physical journal. E, Soft matter · 2023Article
- Review
- Involvement of plasma membrane HPlanta · 2021Article
- The Oligomeric State of the Plasma Membrane H⁺-ATPase fromMolecules (Basel, Switzerland) · 2019Article
- Article
- Nanodisc-based kinetic assays reveal distinct effects of phospholipid headgroups on the phosphoenzyme transition of sarcoplasmic reticulum CaThe Journal of biological chemistry · 2017Article
- Nanodiscs in Membrane Biochemistry and Biophysics.Chemical reviews · 2017Review
- Antigenic characterization of the human immunodeficiency virus (HIV-1) envelope glycoprotein precursor incorporated into nanodiscs.PloS one · 2017Article
- Nanodiscs for structural and functional studies of membrane proteins.Nature structural & molecular biology · 2016Review
- Direct observation of proton pumping by a eukaryotic P-type ATPase.Science (New York, N.Y.) · 2016Article
- Time-Resolved Investigation of Molecular Components Involved in the Induction of [Formula: see text] High Affinity Transport System in Maize Roots.Frontiers in plant science · 2016Article
- Plant lipid environment and membrane enzymes: the case of the plasma membrane H+-ATPase.Plant cell reports · 2015Review
- Kinetics of the early events of GPCR signalling.FEBS letters · 2014Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasma membrane H(+)-ATPases form a subfamily of P-type ATPases responsible for pumping protons out of cells and are essential for establishing and maintaining the crucial transmembrane proton gradient in plants and fungi. Here, we report the reconstitution of the Arabidopsis thaliana plasma membrane H(+)-ATPase isoform 2 into soluble nanoscale lipid bilayers, also termed nanodiscs. Based on native gel analysis and cross-linking studies, the pump inserts into nanodiscs as a functional monomer. Insertion of the H(+)-ATPase into nanodiscs has the potential to enable structural and functional characterization using techniques normally applicable only for soluble proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.