ArticleMolecular and cellular biology2013
Mechanisms of antisense transcription initiation from the 3' end of the GAL10 coding sequence in vivo.
Article in Molecular and cellular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- UPS writes a new saga of SAGA.Biochimica et biophysica acta. Gene regulatory mechanisms · 2023Review
- Proteasomal Regulation of Mammalian SPT16 in Controlling Transcription.Molecular and cellular biology · 2021Article
- An F-Box Protein, Mdm30, Interacts with TREX Subunit Sub2 To Regulate Cellular Abundance Cotranscriptionally in Orchestrating mRNA Export Independently of Splicing and Mitochondrial Function.Molecular and cellular biology · 2020Article
- The nucleosome core particle remembers its position through DNA replication and RNA transcription.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Distinct Functions of the Cap-Binding Complex in Stimulation of Nuclear mRNA Export.Molecular and cellular biology · 2019Article
- Mechanisms of Antisense Transcription Initiation with Implications in Gene Expression, Genomic Integrity and Disease Pathogenesis.Non-coding RNA · 2019Review
- TOR Facilitates the Targeting of the 19S Proteasome Subcomplex To Enhance Transcription Complex Assembly at the Promoters of the Ribosomal Protein Genes.Molecular and cellular biology · 2018Article
- Two Distinct Regulatory Mechanisms of Transcriptional Initiation in Response to Nutrient Signaling.Genetics · 2018Article
- Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast.Scientific reports · 2017Article
- An mRNA Capping Enzyme Targets FACT to the Active Gene To Enhance the Engagement of RNA Polymerase II into Transcriptional Elongation.Molecular and cellular biology · 2017Article
- Fine-Tuning of FACT by the Ubiquitin Proteasome System in Regulation of Transcriptional Elongation.Molecular and cellular biology · 2016Article
- Regulation of Antisense Transcription by NuA4 Histone Acetyltransferase and Other Chromatin Regulatory Factors.Molecular and cellular biology · 2016Article
- Characteristics of Antisense Transcript Promoters and the Regulation of Their Activity.International journal of molecular sciences · 2015Review
- Single-Molecule Imaging Reveals a Switch between Spurious and Functional ncRNA Transcription.Molecular cell · 2015Article
- Eaf1p Is Required for Recruitment of NuA4 in Targeting TFIID to the Promoters of the Ribosomal Protein Genes for Transcriptional Initiation In Vivo.Molecular and cellular biology · 2015Article
- Rrd1p, an RNA polymerase II-specific prolyl isomerase and activator of phosphoprotein phosphatase, promotes transcription independently of rapamycin response.Nucleic acids research · 2014Article
- A new regulatory pathway of mRNA export by an F-box protein, Mdm30.RNA (New York, N.Y.) · 2014Article
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3 authors.
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Abstract
In spite of the important regulatory functions of antisense transcripts in gene expression, it remains unknown how antisense transcription is initiated. Recent studies implicated RNA polymerase II in initiation of antisense transcription. However, how RNA polymerase II is targeted to initiate antisense transcription has not been elucidated. Here, we have analyzed the association of RNA polymerase II with the antisense initiation site at the 3' end of the GAL10 coding sequence in dextrose-containing growth medium that induces antisense transcription. We find that RNA polymerase II is targeted to the antisense initiation site at GAL10 by Reb1p activator as well as general transcription factors (e.g., TFIID, TFIIB, and Mediator) for antisense transcription initiation. Intriguingly, while GAL10 antisense transcription is dependent on TFIID, its sense transcription does not require TFIID. Further, the Gal4p activator that promotes GAL10 sense transcription is dispensable for antisense transcription. Moreover, the proteasome that facilitates GAL10 sense transcription does not control its antisense transcription. Taken together, our results reveal that GAL10 sense and antisense transcriptions are regulated differently and shed much light on the mechanisms of antisense transcription initiation.
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