ArticleJournal of bacteriology2013
Solitary BioY proteins mediate biotin transport into recombinant Escherichia coli.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Exploring the Link Between Biotin Metabolism andPathogens (Basel, Switzerland) · 2026Article
- Arvimicrobium flavum gen. nov., sp. nov., A Novel Genus in the Family Phyllobacteriaceae Isolated From Forest Soil.Current microbiology · 2024Article
- The S-component fold: a link between bacterial transporters and receptors.Communications biology · 2024Article
- Identification of inhibitors targeting the energy-coupling factor (ECF) transporters.Communications biology · 2023Article
- Distribution, abundance, and ecogenomics of themSystems · 2023Article
- The Influence of Genes on the "Killer Plasmid" ofFrontiers in microbiology · 2021Article
- Comparative genomics of the genusMicrobial genomics · 2020Article
- Article
- Chemical Optimization of Whole-Cell Transfer Hydrogenation Using Carbonic Anhydrase as Host Protein.ACS catalysis · 2019Article
- Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM.Nature communications · 2018Article
- Underlying mechanisms for syntrophic metabolism of essential enzyme cofactors in microbial communities.The ISME journal · 2017Article
- The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures.Scientific reports · 2017Article
- The Escherichia coli COG1738 Member YhhQ Is Involved in 7-Cyanodeazaguanine (preQ₀) Transport.Biomolecules · 2017Article
- Article
- Crystal Structure of a Group I Energy Coupling Factor Vitamin Transporter S Component in Complex with Its Cognate Substrate.Cell chemical biology · 2016Article
- Structural insight in the toppling mechanism of an energy-coupling factor transporter.Nature communications · 2016Article
- ATP-dependent Conformational Changes Trigger Substrate Capture and Release by an ECF-type Biotin Transporter.The Journal of biological chemistry · 2015Article
- Structure of a pantothenate transporter and implications for ECF module sharing and energy coupling of group II ECF transporters.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Structural and mechanistic insights into prokaryotic energy-coupling factor transporters.Nature reviews. Microbiology · 2014Article
- EnterohemorrhagicGut microbesArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Energy-coupling factor (ECF) transporters form a large group of vitamin uptake systems in prokaryotes. They are composed of highly diverse, substrate-specific, transmembrane proteins (S units), a ubiquitous transmembrane protein (T unit), and homo- or hetero-oligomeric ABC ATPases. Biotin transporters represent a special case of ECF-type systems. The majority of the biotin-specific S units (BioY) is known or predicted to interact with T units and ABC ATPases. About one-third of BioY proteins, however, are encoded in organisms lacking any recognizable T unit. This finding raises the question of whether these BioYs function as transporters in a solitary state, a feature ascribed to certain BioYs in the past. To address this question in living cells, an Escherichia coli K-12 derivative deficient in biotin synthesis and devoid of its endogenous high-affinity biotin transporter was constructed as a reference strain. This organism is particularly suited for this purpose because components of ECF transporters do not naturally occur in E. coli K-12. The double mutant was viable in media containing either high levels of biotin or a precursor of the downstream biosynthetic path. Importantly, it was nonviable on trace levels of biotin. Eight solitary bioY genes of proteobacterial origin were individually expressed in the reference strain. Each of the BioYs conferred biotin uptake activity on the recombinants, which was inferred from uptake assays with [(3)H]biotin and growth of the cells on trace levels of biotin. The results underscore that solitary BioY transports biotin across the cytoplasmic membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.