ArticleJournal of bacteriology2013
Role of autofluorescence in flow cytometric analysis of Escherichia coli treated with bactericidal antibiotics.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 88 citations in OpenAlex.
- Implications of bacterial exposure to reactive oxygen and chlorine species.Infection and immunity · 2026Review
- Copper biosorption by Serratia plymuthica: crucial role of tightly bound extracellular polymeric substances in planktonic and biofilm systems.Biodegradation · 2026Article
- Fluorescein-based dyes are not valid reporters of oxidative stress in bacteria, and conclusions based on their use must be reconsidered.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Investigating the influence of diverse treatments on the autofluorescence properties of Bacillus subtilis.Scientific reports · 2025Article
- A Label-Free Optical Flow Cytometry Based-Method for Rapid Assay of Disinfectants' Bactericidal Activity.International journal of molecular sciences · 2024Article
- Fluorescent reporters give new insights into antibiotics-induced nonsense and frameshift mistranslation.Scientific reports · 2024Article
- Bacterial metabolites influence the autofluorescence ofFrontiers in microbiology · 2024Article
- Relationship between antimicrobial peptides-induced cell membrane damage and bactericidal activity.Biophysical journal · 2023Article
- Probing Polarity and pH Sensitivity of Carbon Dots inNanomaterials (Basel, Switzerland) · 2023Article
- Article
- Copper-Based Compounds against Erwinia amylovora: Response Parameter Analysis and Suppression of Fire Blight in Apple.The plant pathology journal · 2023Article
- Single-cell scattering and auto-fluorescence-based fast antibiotic susceptibility testing for gram-negative and gram-positive bacteria.Frontiers in microbiology · 2023Article
- Automated analysis of mitochondrial dimensions in mesenchymal stem cells: Current methods and future perspectives.Heliyon · 2023Review
- FISH-TAMB, a Fixation-Free mRNA Fluorescent Labeling Technique to Target Transcriptionally Active Members in Microbial Communities.Microbial ecology · 2022Article
- Oxidative Damage Blocks Thymineless Death and Trimethoprim Poisoning in Escherichia coli.Journal of bacteriology · 2022Article
- An LC-MS/MS assay and complementary web-based tool to quantify and predict compound accumulation in E. coli.Nature protocols · 2021Review
- The Universally Conserved ATPase YchF Regulates Translation of Leaderless mRNA in Response to Stress Conditions.Frontiers in molecular biosciences · 2021Article
- Formulation of pH-Responsive Quatsomes from Quaternary Bicephalic Surfactants and Cholesterol for Enhanced Delivery of Vancomycin against Methicillin ResistantPharmaceutics · 2020Article
- Recent Developments in the Application of Flow Cytometry to Advance our Understanding of Mycobacterium tuberculosis Physiology and Pathogenesis.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Review
- Gamblers: An Antibiotic-Induced Evolvable Cell Subpopulation Differentiated by Reactive-Oxygen-Induced General Stress Response.Molecular cell · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bactericidal antibiotics kill by different mechanisms as a result of a specific interaction with their cellular targets. Over the past few years, alternative explanations for cidality have been proposed based on a postulated common pathway, depending on the intracellular production of reactive oxygen species. Detection of hydroxyl radicals relies on staining with specific fluorescent dyes that can penetrate the cell and are detected using flow cytometry. Flow cytometry has become an important tool in microbiology to study characteristics of individual cells within large heterogeneous cellular populations. We show here that Escherichia coli treated with different bactericidal antibiotics exhibits increased autofluorescence when analyzed by flow cytometry. We present evidence suggesting that this change in autofluorescence is caused by altered cell morphology upon antibiotic treatment. Consistent with this view, mutant cells that fail to elongate upon norfloxacin treatment show no increased auto-fluorescence response. Finally, we present data demonstrating that changes in autofluorescence can impact the results with fluorescent probes when using flow cytometry and confound the findings obtained with specific dyes. In summary, recent findings that correlate the exposure to cidal antibiotics with the production of reactive oxygen species need to be reconsidered in light of such changes in autofluorescence. Conclusive evidence for an increase of hydroxyl radicals after treatment with such drugs is still missing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.