ArticleJournal of bacteriology2013
MrpA functions in energy conversion during acetate-dependent growth of Methanosarcina acetivorans.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Magnetite drives microbial community restructuring and stimulates aceticlastic methanogenesis of type II Methanosarcina in mangrove sediments.Microbiome · 2025Article
- Physiological potential and evolutionary trajectories of syntrophic sulfate-reducing bacterial partners of anaerobic methanotrophic archaea.PLoS biology · 2023Article
- Supplementation of Sulfide or Acetate and 2-Mercaptoethane Sulfonate Restores Growth of theMicroorganisms · 2023Article
- Article
- Review
- Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the GenusMicrobiology and molecular biology reviews : MMBR · 2019Review
- Life on the thermodynamic edge: Respiratory growth of an acetotrophic methanogen.Science advances · 2019Article
- A biochemical framework for anaerobic oxidation of methane driven by Fe(III)-dependent respiration.Nature communications · 2018Article
- Electron Bifurcation and Confurcation in Methanogenesis and Reverse Methanogenesis.Frontiers in microbiology · 2018Review
- Article
- Functional Role of MrpA in the MrpABCDEFG Na+/H+ Antiporter Complex from the Archaeon Methanosarcina acetivorans.Journal of bacteriology · 2017Article
- Mrp Antiporters Have Important Roles in Diverse Bacteria and Archaea.Frontiers in microbiology · 2017Review
- Assessing methanotrophy and carbon fixation for biofuel production by Methanosarcina acetivorans.Microbial cell factories · 2016Article
- Rerouting Cellular Electron Flux To Increase the Rate of Biological Methane Production.Applied and environmental microbiology · 2015Article
- Acetate Metabolism in Anaerobes from the Domain Archaea.Life (Basel, Switzerland) · 2015Review
- Purification and functional reconstitution of a seven-subunit mrp-type na+/h+ antiporter.Journal of bacteriology · 2014Article
- Genetic resources for methane production from biomass described with the Gene Ontology.Frontiers in microbiology · 2014Article
- Characterization of the RnfB and RnfG subunits of the Rnf complex from the archaeon Methanosarcina acetivorans.PloS one · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of the multisubunit sodium/proton antiporter (Mrp) of Methanosarcina acetivorans was investigated with a mutant deleted for the gene encoding the MrpA subunit. Antiporter activity was 5-fold greater in acetate-grown versus methanol-grown wild-type cells, consistent with the previously published relative levels of mrp transcript. The rate, final optical density, and dry weight/methane ratio decreased for the mutant versus wild type when cultured with a growth-limiting concentration of acetate. All growth parameters of the mutant or wild type were identical when grown with methanol in medium containing a growth-limiting Na(+) concentration of 1.04 M. The lag phase, growth rate, and final optical density for growth of the mutant were suboptimal compared to the wild type when cultured with acetate in medium containing either 0.54 or 1.04 M Na(+). The addition of 25 mM NaCl to resting cell suspensions stimulated ATP synthesis driven by a potassium diffusion potential. ATP synthesis was greater in wild-type than mutant cells grown with acetate, a trend that held for methanol-grown cells, albeit less pronounced. Both sodium and proton ionophores reduced ATP synthesis in the wild type grown with either substrate. The results indicated that the Mrp complex is essential for efficient ATP synthesis and optimal growth at the low concentrations of acetate encountered in the environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.