ArticleProceedings of the National Academy of Sciences of the United States of America2013
Stochastic expression and epigenetic memory at the yeast HO promoter.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 27 citations in OpenAlex.
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- Modulators of MAPK pathway activity during filamentous growth in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2024Article
- Review
- Variation, Variegation and Heritable Gene Repression inFrontiers in genetics · 2021Review
- Ash1 and Tup1 dependent repression of the Saccharomyces cerevisiae HO promoter requires activator-dependent nucleosome eviction.PLoS genetics · 2020Article
- Capturing and Understanding the Dynamics and Heterogeneity of Gene Expression in the Living Cell.International journal of molecular sciences · 2020Review
- Article
- Existence, Transition, and Propagation of Intermediate Silencing States in Ribosomal DNA.Molecular and cellular biology · 2019Article
- Using time-lapse fluorescence microscopy to study gene regulation.Methods (San Diego, Calif.) · 2019Review
- Systematic Study of Nucleosome-Displacing Factors in Budding Yeast.Molecular cell · 2018Article
- Transgenerational epigenetic inheritance in birds.Environmental epigenetics · 2018Review
- Construction of hybrid regulated mother-specific yeast promoters for inducible differential gene expression.PloS one · 2018Article
- Disruption of promoter memory by synthesis of a long noncoding RNA.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Interallelic interaction and gene regulation in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Regulation of cell-to-cell variability in divergent gene expression.Nature communications · 2016Article
- Uncoupling histone turnover from transcription-associated histone H3 modifications.Nucleic acids research · 2015Article
- The Rts1 regulatory subunit of PP2A phosphatase controls expression of the HO endonuclease via localization of the Ace2 transcription factor.The Journal of biological chemistry · 2014Article
- Adaptive imaging cytometry to estimate parameters of gene networks models in systems and synthetic biology.PloS one · 2014Article
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9 authors at 3 institutions in 1 country.
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Abstract
Eukaryotic gene regulation usually involves sequence-specific transcription factors and sequence-nonspecific cofactors. A large effort has been made to understand how these factors affect the average gene expression level among a population. However, little is known about how they regulate gene expression in individual cells. In this work, we address this question by mutating multiple factors in the regulatory pathway of the yeast HO promoter (HOpr) and probing the corresponding promoter activity in single cells using time-lapse fluorescence microscopy. We show that the HOpr fires in an "on/off" fashion in WT cells as well as in different genetic backgrounds. Many chromatin-related cofactors that affect the average level of HO expression do not actually affect the firing amplitude of the HOpr; instead, they affect the firing frequency among individual cell cycles. With certain mutations, the bimodal expression exhibits short-term epigenetic memory across the mitotic boundary. This memory is propagated in "cis" and reflects enhanced activator binding after a previous "on" cycle. We present evidence that the memory results from slow turnover of the histone acetylation marks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.