ArticleAntimicrobial agents and chemotherapy2013
Genetic basis for in vitro and in vivo resistance to lincosamides, streptogramins A, and pleuromutilins (LSAP phenotype) in Enterococcus faecium.
Article in Antimicrobial agents and chemotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 54 citations in OpenAlex.
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- Genomic surveillance of vancomycin-resistant Enterococcus faecium: a study on Resistome, Plasmidome, and mobilome profiling.Current genetics · 2025Article
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- Genome-based taxonomic identification and safety assessment of an Enterococcus strain isolated from a homemade dairy product.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
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- CRISPR-Cas Systems in the Fight Against Antimicrobial Resistance: Current Status, Potentials, and Future Directions.Infection and drug resistance · 2024Review
- Investigating antimicrobial resistance genes in probiotic products for companion animals.Frontiers in veterinary science · 2024Article
- Phylogeny, Virulence, and Antimicrobial Resistance Gene Profiles ofAntibiotics (Basel, Switzerland) · 2023Article
- Genomic insights into antibiotic resistance and mobilome of lactic acid bacteria and bifidobacteria.Life science alliance · 2023Article
- An in-house 45-plex array for the detection of antimicrobial resistance genes in Gram-positive bacteria.MicrobiologyOpen · 2023Article
- Dissemination of Quinupristin-Dalfopristin and Linezolid resistance genes among hospital environmental and healthy volunteer fecal isolates of Enterococcus faecalis and Enterococcus faecium.Molecular biology reports · 2022Article
- Safety and efficacy of a feed additive consisting ofEFSA journal. European Food Safety Authority · 2022Article
- Mobile Antimicrobial Resistance Genes in Probiotics.Antibiotics (Basel, Switzerland) · 2021Article
- From the Farms to the Dining Table: The Distribution and Molecular Characteristics of Antibiotic-ResistantMicroorganisms · 2021Article
- Surveillance of Enterococcus spp. reveals distinct species and antimicrobial resistance diversity across a One-Health continuum.Scientific reports · 2020Article
- Phenotypic and Genotypic Traits of Vancomycin-Resistant Enterococci from Healthy Food-Producing Animals.Microorganisms · 2020Article
- How CRISPR-Cas System Could Be Used to Combat Antimicrobial Resistance.Infection and drug resistance · 2020Review
- Emergence of an Australian-like pstS-null vancomycin resistant Enterococcus faecium clone in Scotland.PloS one · 2019Article
- The use of high-throughput sequencing to investigate an outbreak of glycopeptide-resistant Enterococcus faecium with a novel quinupristin-dalfopristin resistance mechanism.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
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4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As opposed to Enterococcus faecalis, which is intrinsically resistant to lincosamides, streptogramins A, and pleuromutilins (LSAP phenotype) by production of the ABC protein Lsa(A), Enterococcus faecium is naturally susceptible. Since this phenotype may be selected for in vivo by quinupristin-dalfopristin (Q-D), the aim of this study was to investigate the molecular mechanism of acquired LSAP resistance in E. faecium. Six LSAP-resistant in vitro mutants of E. faecium HM1070 as well as three different pairs of clinical isolates (pre- and postexposure to Q-D) were studied. The full genome sequence of an in vitro mutant (E. faecium UCN90B) was determined by using 454 sequencing technology and was compared with that of the parental strain. Single-nucleotide replacement was carried out to confirm the role of this mutation. By comparative genomic analysis, a point mutation was found within a 1,503-bp gene coding for an ABC homologue showing 66% amino acid identity with Lsa(A). This mutation (C1349T) led to an amino acid substitution (Thr450Ile). An identical mutation was identified in all in vitro and in vivo resistant strains but was not present in susceptible strains. The wild-type allele was named eat(A) (for Enterococcus ABC transporter), and its mutated allelic variant was named eat(A)v. The introduction of eat(A)v from UCN90B into HM1070 conferred the LSAP phenotype, whereas that of eat(A) from HM1070 into UCN90B restored susceptibility entirely. This is the first description of the molecular mechanism of acquired LSAP resistance in E. faecium. Characterization of the biochemical mechanism of resistance and the physiological role of this ABC protein need further investigations.
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