ArticleJournal of industrial microbiology & biotechnology2013
Metabolic engineering of Corynebacterium glutamicum for increasing the production of L-ornithine by increasing NADPH availability.
Article in Journal of industrial microbiology & biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 56 citations in OpenAlex.
- Increased NADPH Supply Enhances Glycolysis Metabolic Flux and L-methionine Production inFoods (Basel, Switzerland) · 2022Article
- Enhanced L-ornithine production from glucose and sucrose via manipulation of the fructose metabolic pathway in Corynebacterium glutamicum.Bioresources and bioprocessing · 2022Article
- Multifunctional phototheranostic agent ZnO@Ag for anti-infection through photothermal/photodynamic therapy.Frontiers in chemistry · 2022Article
- Production of l-glutamate family amino acids inSynthetic and systems biotechnology · 2021Review
- Proteome analysis guided genetic engineering of Corynebacterium glutamicum S9114 for tween 40-triggered improvement in L-ornithine production.Microbial cell factories · 2020Article
- Improvement of l-Leucine Production inInternational journal of molecular sciences · 2019Article
- Equilibrium of the intracellular redox state for improving cell growth and L-lysine yield of Corynebacterium glutamicum by optimal cofactor swapping.Microbial cell factories · 2019Article
- Recent Advances of L-ornithine Biosynthesis in Metabolically EngineeredFrontiers in bioengineering and biotechnology · 2019Review
- Improving squalene production by enhancing the NADPH/NADPBiotechnology for biofuels · 2019Article
- Optimization of ʟ-ornithine production in recombinant Corynebacterium glutamicum S9114 by cg3035 overexpression and manipulating the central metabolic pathway.Microbial cell factories · 2018Article
- Improved L-ornithine production in Corynebacterium crenatum by introducing an artificial linear transacetylation pathway.Journal of industrial microbiology & biotechnology · 2018Article
- Redox cofactor engineering in industrial microorganisms: strategies, recent applications and future directions.Journal of industrial microbiology & biotechnology · 2018Review
- Metabolic evolution and a comparative omics analysis of Corynebacterium glutamicum for putrescine production.Journal of industrial microbiology & biotechnology · 2018Article
- Our microbes not only produce antibiotics, they also overproduce amino acids.The Journal of antibiotics · 2017Review
- Systematic pathway engineering of Corynebacterium glutamicum S9114 for L-ornithine production.Microbial cell factories · 2017Article
- Transcriptomic Changes in Response to Putrescine Production in Metabolically EngineeredFrontiers in microbiology · 2017Article
- Construction of a highly efficient Bacillus subtilis 168 whole-cell biocatalyst and its application in the production of L-ornithine.Journal of industrial microbiology & biotechnology · 2015Article
- Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine.Nature communications · 2015Article
- Production of L-ornithine from sucrose and molasses by recombinant Corynebacterium glutamicum.Folia microbiologica · 2015Article
- Metabolic engineering of an ATP-neutral Embden-Meyerhof-Parnas pathway in Corynebacterium glutamicum: growth restoration by an adaptive point mutation in NADH dehydrogenase.Applied and environmental microbiology · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The experiments presented here were based on the conclusions of our previous proteomic analysis. Increasing the availability of glutamate by overexpression of the genes encoding enzymes in the L-ornithine biosynthesis pathway upstream of glutamate and disruption of speE, which encodes spermidine synthase, improved L-ornithine production by Corynebacterium glutamicum. Production of L-ornithine requires 2 moles of NADPH per mole of L-ornithine. Thus, the effect of NADPH availability on L-ornithine production was also investigated. Expression of Clostridium acetobutylicum gapC, which encodes NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, and Bacillus subtilis rocG, which encodes NAD-dependent glutamate dehydrogenase, led to an increase of L-ornithine concentration caused by greater availability of NADPH. Quantitative real-time PCR analysis demonstrates that the increased levels of NADPH resulted from the expression of the gapC or rocG gene rather than that of genes (gnd, icd, and ppnK) involved in NADPH biosynthesis. The resulting strain, C. glutamicum ΔAPRE::rocG, produced 14.84 g l⁻¹ of L-ornithine. This strategy of overexpression of gapC and rocG will be useful for improving production of target compounds using NADPH as reducing equivalent within their synthetic pathways.
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