ArticleJournal of industrial microbiology & biotechnology2013
Moderate expression of the transcriptional regulator ALsR enhances acetoin production by Bacillus subtilis.
Article in Journal of industrial microbiology & biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
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- Anode-assisted electro-fermentation with Bacillus subtilis under oxygen-limited conditions.Biotechnology for biofuels and bioproducts · 2023Article
- Acetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in Bacillus subtilis.Biotechnology for biofuels and bioproducts · 2022Article
- Perception of Biocontrol Potential ofBiology · 2022Article
- Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2020Article
- Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method.Microbial cell factories · 2019Article
- Enhancing 2-Ketogluconate Production ofMolecules (Basel, Switzerland) · 2018Article
- Strategies for efficient and economical 2,3-butanediol production: new trends in this field.World journal of microbiology & biotechnology · 2016Review
- Metabolic engineering ofBioresources and bioprocessing · 2016Article
- Article
- Production of Acetoin through Simultaneous Utilization of Glucose, Xylose, and Arabinose by Engineered Bacillus subtilis.PloS one · 2016Article
- Enhanced 2,3-butanediol production from biodiesel-derived glycerol by engineering of cofactor regeneration and manipulating carbon flux in Bacillus amyloliquefaciens.Microbial cell factories · 2015Article
- Article
- Article
- Efficient bioconversion of 2,3-butanediol into acetoin using Gluconobacter oxydans DSM 2003.Biotechnology for biofuels · 2013Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acetoin, a major extracellular catabolic product of Bacillus subtilis cultured on glucose, is widely used to add flavor to food and also serves as a precursor for chemical synthesis. The biosynthesis of acetoin from pyruvate requires the enzymes α-acetolactate synthase (ALS) and α-acetolactate decarboxylase (ALDC), both of which are encoded by the alsSD operon. The transcriptional regulator ALsR is essential for the expression of alsSD. Here we focused on enhancing the production of acetoin by B. subtilis using different promoters to express ALsR. The expression of reporter genes was much higher under the control of the HpaII promoter than under control of the P bdhA promoter. Although the HpaII promoter highly enhanced transcription of the alsSD operon through overexpression of ALsR, the production of acetoin was not significantly increased. In contrast, moderate enhancement of ALsR expression using the P bdhA promoter significantly improved acetoin production. Compared with the wild-type, the enzyme activities of ALS and ALDC in B. subtilis harboring P bdhA were increased by approximately twofold, and the molar yield of acetoin from glucose was improved by 62.9 % in shake flask fermentation. In a 5-L fermentor, the engineered B. subtilis ultimately yielded 41.5 g/L of acetoin. Based on these results, we conclude that enhanced expression of ALDC and ALS by moderately elevated expression of the transcriptional regulator ALsR could increase acetoin production in recombinant B. subtilis.
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