ArticleBMC biology2013
Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish.
Article in BMC biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 38 citations in OpenAlex.
- Functional genomics analysis of developing zebrafish and human endoderm reveals highly conservedGenome research · 2026Article
- Identification of intestinal enteroendocrine cell subtypes and their associated hormones in zebrafish.PLoS biology · 2025Article
- Mechanistic insights and approaches for beta cell regeneration.Nature chemical biology · 2025Review
- Zebrafish pancreatic β cell clusters undergo stepwise regeneration using Neurod1-expressing cells from different cell lineages.Cell and tissue research · 2023Article
- Decoding pancreatic endocrine cell differentiation and β cell regeneration in zebrafish.Science advances · 2023Article
- Ptf1a function and transcriptional cis-regulation, a cornerstone in vertebrate pancreas development.The FEBS journal · 2022Review
- Identification of an evolutionarily conserved domain in Neurod1 favouring enteroendocrine versus goblet cell fate.PLoS genetics · 2022Article
- Article
- A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Sexually dimorphic roles for the type 2 diabetes-associated C2cd4b gene in murine glucose homeostasis.Diabetologia · 2021Article
- Differential Morphological Diagnosis of Various Forms of Congenital Hyperinsulinism in Children.Frontiers in endocrinology · 2021Article
- Pancreatic and intestinal endocrine cells in zebrafish share common transcriptomic signatures and regulatory programmes.BMC biology · 2020Article
- Inducible Mosaic Cell Labeling Provides Insights Into Pancreatic Islet Morphogenesis.Frontiers in cell and developmental biology · 2020Article
- Single-cell analyses identify distinct and intermediate states of zebrafish pancreatic islet development.Journal of molecular cell biology · 2019Article
- Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling.Development (Cambridge, England) · 2018Article
- Article
- Transcriptome analysis of pancreatic cells across distant species highlights novel important regulator genes.BMC biology · 2017Article
- sept7b is required for the differentiation of pancreatic endocrine progenitors.Scientific reports · 2016Article
- Progenitor potential of nkx6.1-expressing cells throughout zebrafish life and during beta cell regeneration.BMC biology · 2015Article
- Polyamine biosynthesis is critical for growth and differentiation of the pancreas.Scientific reports · 2015Article
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7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNEUROG3 is a key regulator of pancreatic endocrine cell differentiation in mouse, essential for the generation of all mature hormone producing cells. It is repressed by Notch signaling that prevents pancreatic cell differentiation by maintaining precursors in an undifferentiated state.
resultsWe show that, in zebrafish, neurog3 is not expressed in the pancreas and null neurog3 mutant embryos do not display any apparent endocrine defects. The control of endocrine cell fate is instead fulfilled by two basic helix-loop-helix factors, Ascl1b and Neurod1, that are both repressed by Notch signaling. ascl1b is transiently expressed in the mid-trunk endoderm just after gastrulation and is required for the generation of the first pancreatic endocrine precursor cells. Neurod1 is expressed afterwards in the pancreatic anlagen and pursues the endocrine cell differentiation program initiated by Ascl1b. Their complementary role in endocrine differentiation of the dorsal bud is demonstrated by the loss of all hormone-secreting cells following their simultaneous inactivation. This defect is due to a blockage of the initiation of endocrine cell differentiation.
conclusionsThis study demonstrates that NEUROG3 is not the unique pancreatic endocrine cell fate determinant in vertebrates. A general survey of endocrine cell fate determinants in the whole digestive system among vertebrates indicates that they all belong to the ARP/ASCL family but not necessarily to the Neurog3 subfamily. The identity of the ARP/ASCL factor involved depends not only on the organ but also on the species. One could, therefore, consider differentiating stem cells into insulin-producing cells without the involvement of NEUROG3 but via another ARP/ASCL factor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.