ArticleApplied and environmental microbiology2013
Malic enzyme and malolactic enzyme pathways are functionally linked but independently regulated in Lactobacillus casei BL23.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 46 citations in OpenAlex.
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- Nutritional evaluation, characteristic component differences and non-targeted metabolomics analysis of traditional fermented glutinous Rice in four Chinese provinces.Food chemistry: X · 2026Article
- Effect of pre-treated homogenized egg liquid addition on yogurt quality and metabolic profiles.Current research in food science · 2026Article
- Evaluation of Genome-Scale Model Reconstruction Strategies forMetabolites · 2025Article
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- The Effect of Ultrasound and Lactic Acid Fermentation on the Selected Quality Parameters and Bioactive Compounds Content in Fermented Pumpkin (Molecules (Basel, Switzerland) · 2024Article
- Research on Engineering theFoods (Basel, Switzerland) · 2024Article
- Effects of Heterologous Expression of Genes Related L-Malic acid Metabolism inFoods (Basel, Switzerland) · 2024Article
- Optimization of tomato (Frontiers in nutrition · 2024Article
- An underlying mechanism for MleR activating the malolactic enzyme pathway to enhance acid tolerance inApplied and environmental microbiology · 2023Article
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- Transforming Spent Coffee Grounds' Hydrolysates with YeastFoods (Basel, Switzerland) · 2023Article
- Aroma profiles of sweet cherry juice fermented by different lactic acid bacteria determined through integrated analysis of electronic nose and gas chromatography-ion mobility spectrometry.Frontiers in microbiology · 2023Article
- Biovalorization of Market Surplus Bread for Development of Probiotic-Fermented Potential Functional Beverages.Foods (Basel, Switzerland) · 2022Article
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- The Role of Yeasts and Lactic Acid Bacteria on the Metabolism of Organic Acids during Winemaking.Foods (Basel, Switzerland) · 2020Review
- A metabolic reconstruction of Lactobacillus reuteri JCM 1112 and analysis of its potential as a cell factory.Microbial cell factories · 2019Article
- Use of Dairy and Plant-Derived Lactobacilli as Starters for Cherry Juice Fermentation.Nutrients · 2019Article
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4 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Lactobacillus casei is the only lactic acid bacterium in which two pathways for l-malate degradation have been described: the malolactic enzyme (MLE) and the malic enzyme (ME) pathways. Whereas the ME pathway enables L. casei to grow on l-malate, MLE does not support growth. The mle gene cluster consists of three genes encoding MLE (mleS), the putative l-malate transporter MleT, and the putative regulator MleR. The mae gene cluster consists of four genes encoding ME (maeE), the putative transporter MaeP, and the two-component system MaeKR. Since both pathways compete for the same substrate, we sought to determine whether they are coordinately regulated and their role in l-malate utilization as a carbon source. Transcriptional analyses revealed that the mle and mae genes are independently regulated and showed that MleR acts as an activator and requires internalization of l-malate to induce the expression of mle genes. Notwithstanding, both l-malate transporters were required for maximal l-malate uptake, although only an mleT mutation caused a growth defect on l-malate, indicating its crucial role in l-malate metabolism. However, inactivation of MLE resulted in higher growth rates and higher final optical densities on l-malate. The limited growth on l-malate of the wild-type strain was correlated to a rapid degradation of the available l-malate to l-lactate, which cannot be further metabolized. Taken together, our results indicate that L. casei l-malate metabolism is not optimized for utilization of l-malate as a carbon source but for deacidification of the medium by conversion of l-malate into l-lactate via MLE.
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