ArticleMolecular microbiology2013
Peptidoglycan hydrolysis is required for assembly and activity of the transenvelope secretion complex during sporulation in Bacillus subtilis.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Cleavage of an engulfment peptidoglycan hydrolase by a sporulation signature protease in Clostridioides difficile.Molecular microbiology · 2024Article
- To Feed or to Stick? Genomic Analysis Offers Clues for the Role of a Molecular Machine in Endospore Formers.Journal of bacteriology · 2022Review
- Chromosome Segregation and Peptidoglycan Remodeling Are Coordinated at a Highly Stabilized Septal Pore to Maintain Bacterial Spore Development.Developmental cell · 2021Article
- A dynamic, ring-forming MucB / RseB-like protein influences spore shape in Bacillus subtilis.PLoS genetics · 2020Article
- Shaping an Endospore: Architectural Transformations DuringAnnual review of microbiology · 2020Review
- Article
- Sporulation and Germination in Clostridial Pathogens.Microbiology spectrum · 2019Review
- Differential requirements for conserved peptidoglycan remodeling enzymes during Clostridioides difficile spore formation.Molecular microbiology · 2018Article
- Peptidoglycan degradation machinery in Clostridium difficile forespore engulfment.Molecular microbiology · 2018Article
- A two-step transport pathway allows the mother cell to nurture the developing spore in Bacillus subtilis.PLoS genetics · 2017Article
- A ring-shaped conduit connects the mother cell and forespore during sporulation in Bacillus subtilis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- GerM is required to assemble the basal platform of the SpoIIIA-SpoIIQ transenvelope complex during sporulation in Bacillus subtilis.Molecular microbiology · 2016Article
- A Membrane-Embedded Amino Acid Couples the SpoIIQ Channel Protein to Anti-Sigma Factor Transcriptional Repression during Bacillus subtilis Sporulation.Journal of bacteriology · 2016Article
- The SpoIIQ-SpoIIIAH complex of Clostridium difficile controls forespore engulfment and late stages of gene expression and spore morphogenesis.Molecular microbiology · 2016Article
- Salt-sensitivity of σ(H) and Spo0A prevents sporulation of Bacillus subtilis at high osmolarity avoiding death during cellular differentiation.Molecular microbiology · 2016Article
- High-Throughput Genetic Screens Identify a Large and Diverse Collection of New Sporulation Genes in Bacillus subtilis.PLoS biology · 2016Article
- Diverse mechanisms regulate sporulation sigma factor activity in the Firmicutes.Current opinion in microbiology · 2015Review
- Bistable forespore engulfment in Bacillus subtilis by a zipper mechanism in absence of the cell wall.PLoS computational biology · 2014Article
- Stably bridging a great divide: localization of the SpoIIQ landmark protein in Bacillus subtilis.Molecular microbiology · 2013Article
- The SpoIIQ landmark protein has different requirements for septal localization and immobilization.Molecular microbiology · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Sporulating Bacillus subtilis cells assemble a transenvelope secretion complex that connects the mother cell and developing spore. The forespore protein SpoIIQ and the mother-cell protein SpoIIIAH interact across the double membrane septum and are thought to assemble into a channel that serves as the basement layer of this specialized secretion system. SpoIIQ is absolutely required to recruit SpoIIIAH to the sporulation septum on the mother-cell side, however the mechanism by which SpoIIQ is localized has been unclear. Here, we show that SpoIIQ localization requires its partner protein SpoIIIAH and degradation of the septal peptidoglycan (PG) by the two cell wall hydrolases SpoIID and SpoIIP. Our data suggest that PG degradation enables a second mother-cell-produced protein to interact with SpoIIQ. Cells in which both mother-cell anchoring mechanisms have been disabled have a synergistic sporulation defect suggesting that both localization factors function in the secretion complex. Finally, we show that septal PG degradation is critical for the assembly of an active complex. Altogether, these results suggest that the specialized secretion system that links the mother cell and forespore has a complexity approaching those found in Gram-negative bacteria and reveal that the sporulating cell must overcome similar challenges in assembling a transenvelope complex.
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