Evidence map›Paper›PMID 23834383›Full record

ArticleBioTechniques2013

Collection of islets of Langerhans using an equilibrium method.

Duk-Su Koh, Mark Moody, Junghyo Jo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioTechniques, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. An optimized protocol for purification of functional islets of Langerhans.Laboratory investigation; a journal of technical methods and pathology · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Duk-Su KohDepartment of Physiology and Biophysics, University of Washington, Seattle, WA, USA. koh@uw.edu
Mark Moody
Junghyo Jo

Funding

Vector and Transgenic Mouse CoreP30DK017047 · NIDDK · UNIVERSITY OF WASHINGTON · PI Sakeneh Zraika · 1986 to 2026
$41.4M
Electric Studies of Excitation, Secretion & ContractionR01GM083913 · NIGMS · UNIVERSITY OF WASHINGTON · PI HILLE, BERTIL · 2008 to 2020
$4.3M
Trafficking mechanisms for secretory vesicles in pancreatic duct epithelial cellsR01DK080840 · NIDDK · UNIVERSITY OF WASHINGTON · PI KOH, DUK-SU · 2011 to 2015
$1.6M
NIDDK NIH HHS DK017047NIDDK NIH HHS DK080840NIDDK NIH HHS P30 DK017047NIDDK NIH HHS R01 DK080840NIGMS NIH HHS GM83913NIGMS NIH HHS R01 GM083913
6 · The paper itself

Abstract

Here we present a convenient method for easy hand selection of enzymatically isolated small tissues such as islets of Langerhans. Islets are continuously collected in a micropipette tip connected to a peristaltic pump. After entering the conical micropipette tip, the islets are quickly dragged up by solution flow, but this movement subsequently decreases as the flow rate decreases. Thus, the islets are trapped at a specific height where downward gravitation balances upward buoyancy and the drag provided by solution flow. Our device allows more efficient isolation of islets compared to conventional manual collection methods.

Indexed as

AnimalsCell SeparationHumansInsulinIslets of LangerhansMicePancreasInsulin

Identifiers

PMID23834383
PMCPMC3821530

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.